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首页> 外文期刊>Bioprocess and Biosystems Engineering >Selective recovery of acidic and lactonic sophorolipids from culture broths towards the improvement of their therapeutic potential
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Selective recovery of acidic and lactonic sophorolipids from culture broths towards the improvement of their therapeutic potential

机译：从培养液中选择性回收酸性和乳酸槐糖脂，以提高其治疗潜力

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摘要

Sophorolipids (SLs) were produced by Starmerella bombicola. The separation and purification of SLs are a complex process, since they are produced as a mixture of compounds with few structural differences. Solvent extraction is commonly used in downstream processing. In this work, an environmental friendly approach was developed for SLs recovery and purification, based on neutral polymeric sorbents, Amberlite XAD16N(TM), XAD18(TM), and XAD1600N(TM). In batch microassays, key parameters of sorption/desorption process (e.g., contact time, temperature, sorbents, and SLs concentrations) were optimized for separation of acidic and lactonic SLs. Sorption equilibrium was reached after 2-3 h, for all the sorbents tested. Among them XAD1600N(TM) showed a higher sorption capacity (q (max) 230 mg g(-1)), a higher removal (ae100 %) of acidic and lactonic SLs [1 and 2.5 % (w/v)], and the best selectivity. Methanol, ethanol, and acetone were suitable for SLs elution. A selective desorption of SLs was attained with acetonitrile aqueous solutions (v/v): (1) 25 % led to 88.3 % of acidic SLs and (2) 55 % followed by methanol solution (100 %) led to 93.2 % of purified lactonic SLs. This achievement was particularly important regarding SLs potential therapeutic applications, since acidic and lactonic SLs show different biologic activities. In fact, acid SLs show higher virucidal and pro-inflammatory cytokine activity, while lactonic SLs show stronger spermicidal and anti-cancer activity.
机译：槐糖脂（SLs）由Starmerella bombicola生产。 SL的分离和纯化是一个复杂的过程，因为它们是以几乎没有结构差异的化合物混合物的形式生产的。在下游加工中通常使用溶剂萃取。在这项工作中，基于中性聚合物吸附剂，Amberlite XAD16N（TM），XAD18（TM）和XAD1600N（TM），开发了一种环保的方法用于SL的回收和纯化。在分批微量分析中，吸附/解吸过程的关键参数（例如接触时间，温度，吸附剂和SLs浓度）已优化用于分离酸性SLs和内酯SLs。对于所有测试的吸附剂，在2-3小时后达到吸附平衡。其中XAD1600N（TM）表现出更高的吸附容量（q（最大）230 mg g（-1）），酸性和内酯SLs的去除率（ae100％）[1和2.5％（w / v）]，以及最佳选择性。甲醇，乙醇和丙酮适用于SLs洗脱。用乙腈水溶液（v / v）选择性地解吸SLs：（1）25％生成88.3％的酸性SLs，（2）55％随后是甲醇溶液（100％）生成93.2％的纯化内酯SL。对于酸性SLs潜在的治疗应用而言，这一成就尤其重要，因为酸性和内酯SLs表现出不同的生物活性。实际上，酸性SL表现出更高的杀病毒和促炎细胞因子活性，而内酯SL表现出更强的杀精和抗癌活性。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2016年第12期|1825-1837|共13页
  • 作者

    Ribeiro Isabel A. C.; Rosario Bronze M.; Castro Matilde F.; Ribeiro Maria H. L.;

  • 作者单位

    Univ Lisbon, Res Inst Med IMedULisboa, Fac Pharm, Ave Prof Gama Pinto, P-1649003 Lisbon, Portugal|Univ Lisbon, Fac Pharm, Ave Prof Gama Pinto, P-1649003 Lisbon, Portugal;

    Univ Lisbon, Fac Pharm, Ave Prof Gama Pinto, P-1649003 Lisbon, Portugal|Inst Tecnol Quim & Biol, Apartado 127, P-2784505 Oeiras, Portugal;

    Univ Lisbon, Res Inst Med IMedULisboa, Fac Pharm, Ave Prof Gama Pinto, P-1649003 Lisbon, Portugal|Univ Lisbon, Fac Pharm, Ave Prof Gama Pinto, P-1649003 Lisbon, Portugal;

    Univ Lisbon, Res Inst Med IMedULisboa, Fac Pharm, Ave Prof Gama Pinto, P-1649003 Lisbon, Portugal|Univ Lisbon, Fac Pharm, Ave Prof Gama Pinto, P-1649003 Lisbon, Portugal;

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  • 关键词

    Acidic and lactonic sophorolipids; Starmerella bombicola; Sorption; Neutral polymeric resins;

    机译：酸性和内酯的槐糖脂;小球藻;吸附;中性聚合物树脂;

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