...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Bioprocess and Biosystems Engineering >Acetylcholinesterase biosensor based on multi-walled carbon nanotubes-SnO_2-chitosan nanocomposite
【24h】

Acetylcholinesterase biosensor based on multi-walled carbon nanotubes-SnO_2-chitosan nanocomposite

机译：基于多壁碳纳米管-SnO_2-壳聚糖纳米复合材料的乙酰胆碱酯酶生物传感器

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A sensitive amperometric acetylcholinesterase (AChE) biosensor was developed based on the nanocomposite of multi-walled carbon nanotubes (MWCNTs), tin oxide (SnO_2) nanoparticles and chitosan (CHIT). Acetylcholinesterase (AChE) and Nafion were immobilized onto the nanocomposite film to prepare AChE biosensor for pesticide residues detection. The morphologies and electrochemistry properties of the surface modification were investigated using cyclic voltammetry, differential pulse voltammetry, and scanning electron microscopy, respectively. Compared with individual MWCNTs-CHIT, SnO_2-CHIT and bare gold electrode, this nanocomposite showed the most obvious electrochemical signal in the presence of [Fe(CN)_6]~(3-/4-) as a redox couple. Incorporating MWCNTs and SnO_2 into 0.2 % CHIT solution can promote electron transfer, enhance the electrochemical response, and improve the microarchitecture of the electrode surface. All variables involved in the preparation process and analytical performance of the biosensor were optimized. Under optimized conditions, the AChE biosensor exhibited a wide linear range from 0.05 to 1.0 × 10~5 μg/L and with a detection limit for chlorpyrifos was 0.05 μg/L. Based on the inhibition of pesticides on the AChE activity, using chlorpyrifos as model pesticide, the proposed biosensor exhibited a wide range, low detection limit, good reproducibility, and high stability. Using cabbages, lettuces, leeks, and pakchois as model samples, acceptable recovery of 98.7-105.2 % was obtained. The proposed method was proven to be a feasible quantitative method for chlorpyrifos analysis, which may open a new door ultrasensitive detection of chlorpyrifos residues in vegetables and fruits.
机译：基于多壁碳纳米管（MWCNT），氧化锡（SnO_2）纳米粒子和壳聚糖（CHIT）的纳米复合材料，开发了一种灵敏的乙酰胆碱酯酶（AChE）生物传感器。将乙酰胆碱酯酶（AChE）和Nafion固定在纳米复合膜上，以制备用于农药残留检测的AChE生物传感器。分别使用循环伏安法，微分脉冲伏安法和扫描电子显微镜研究了表面改性的形貌和电化学性质。与单独的MWCNTs-CHIT，SnO_2-CHIT和裸金电极相比，该纳米复合材料在以[Fe（CN）_6]〜（3- / 4-）作为氧化还原对存在下表现出最明显的电化学信号。将MWCNT和SnO_2掺入0.2％CHIT溶液中可以促进电子转移，增强电化学响应，并改善电极表面的微结构。优化了生物传感器的制备过程和分析性能中的所有变量。在最佳条件下，AChE生物传感器的线性范围从0.05到1.0×10〜5μg/ L，毒死rif的检出限为0.05μg/ L。基于毒死rif对农药的抑制作用，以毒死rif为模型农药，该生物传感器具有范围广，检测限低，重现性好，稳定性高等特点。使用卷心菜，生菜，韭菜和小白菜作为模型样品，回收率达到98.7-105.2％。所提出的方法被证明是一种可行的定量分析毒死may的方法，可能为蔬菜和水果中毒死rif残留的超灵敏检测打开新的大门。

著录项

  • 来源
    《Bioprocess and Biosystems Engineering》 |2015年第2期|315-321|共7页
  • 作者

    Dongfei Chen; Xia Sun; Yemin Guo; Lu Qiao; Xiangyou Wang;

  • 作者单位

    School of Agriculture and Food Engineering, Shandong University of Technology, NO. 12, Zhangzhou Road, Zibo 255049, Shandong Province, People's Republic of China;

    School of Agriculture and Food Engineering, Shandong University of Technology, NO. 12, Zhangzhou Road, Zibo 255049, Shandong Province, People's Republic of China;

    School of Agriculture and Food Engineering, Shandong University of Technology, NO. 12, Zhangzhou Road, Zibo 255049, Shandong Province, People's Republic of China;

    School of Agriculture and Food Engineering, Shandong University of Technology, NO. 12, Zhangzhou Road, Zibo 255049, Shandong Province, People's Republic of China;

    School of Agriculture and Food Engineering, Shandong University of Technology, NO. 12, Zhangzhou Road, Zibo 255049, Shandong Province, People's Republic of China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Biosensor; Acetylcholinesterase; Multi-walled carbon nanotubes; Tin oxide;

    机译：生物传感器乙酰胆碱酯酶;多壁碳纳米管;氧化锡;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号