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首页> 外文期刊>Bioprocess and Biosystems Engineering >Mandelic acid chiral separation utilizing a two-phase partitioning bioreactor built by polysulfone microspheres and immobilized enzymes
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Mandelic acid chiral separation utilizing a two-phase partitioning bioreactor built by polysulfone microspheres and immobilized enzymes

机译：利用聚砜微球和固定化酶构建的两相分配生物反应器进行扁桃酸手性分离

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摘要

A novel two-phase partitioning bioreactor (TPPB) modified by polysulfone (PSF) microspheres and immobilized enzyme (novozym-435) was formed, and the resulting TPPB was applied into mandelic acid chiral separation. The PSF microspheres containing n-hexanol (named PSF/hexanol microspheres) was prepared by using the phase inversion method, which was used as the organic phase. Meanwhile, the immobilized enzyme novozym-435 was used as a biocatalyst. The water phase was composed of the phosphate buffer solution (PBS). (R, S)-Methyi mandelate was selected as the substrate to study enzymatic properties. Different reaction factors have been researched, such as pH, reaction time, temperature and the quantity of biocatalyst and PSF/hexanol microspheres added in. Finally, (S)-mandelic acid was obtained with an 80 % optical purity after 24 h in the two-phase partitioning bioreactor. The enantiomeric excess (ee_p) values were very low in the water phase, in which the highest ee_p value was only 46 %. The ee_p of the two-phase partitioning bioreactor had been enhanced more obviously than that catalyzed in the water phase.
机译：形成了由聚砜（PSF）微球和固定化酶（novozym-435）修饰的新型两相分配生物反应器（TPPB），并将其应用于扁桃酸手性分离中。通过使用相转化法制备含有正己醇的PSF微球（称为PSF /己醇微球），将其用作有机相。同时，使用固定化酶novozym-435作为生物催化剂。水相由磷酸盐缓冲溶液（PBS）组成。选择（R，S）-扁桃酸甲替基酯作为底物以研究酶学性质。研究了不同的反应因素，例如pH，反应时间，温度以及所添加的生物催化剂和PSF /己醇微球的数量。最后，在两种条件下，经过24小时后，得到的（S）-扁桃酸的光学纯度为80％。相分配生物反应器。在水相中，对映体过量（ee_p）值非常低，其中最高ee_p值仅为46％。两相分配生物反应器的ee_p比水相催化的增强。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2015年第3期|429-435|共7页
  • 作者

    Xinyu Wang; Yanjun Cui; Xia Chen; Hao Zhu; Weiwei Zhu; Yanfeng Li;

  • 作者单位

    State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Institute of Biochemical Engineering and Environmental Technology, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China;

    State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Institute of Biochemical Engineering and Environmental Technology, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China,Lanzhou Petrochemical Research Center of PetroChina, Lanzhou 730060, China;

    State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Institute of Biochemical Engineering and Environmental Technology, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China;

    State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Institute of Biochemical Engineering and Environmental Technology, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China;

    State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Institute of Biochemical Engineering and Environmental Technology, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China;

    State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Institute of Biochemical Engineering and Environmental Technology, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Chiral resolution; Two-phase partitioning bioreactor; Polysulfone microspheres; Novozym-435; (S)-Mandelic acid;

    机译：手性拆分;两相分配生物反应器;聚砜微球;Novozym-435;（S）-扁桃酸;

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