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首页> 外文期刊>Bioprocess and Biosystems Engineering >Characterization of salt-tolerant beta-glucosidase with increased thermostability under high salinity conditions from Bacillus sp SJ-10 isolated from jeotgal, a traditional Korean fermented seafood
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Characterization of salt-tolerant beta-glucosidase with increased thermostability under high salinity conditions from Bacillus sp SJ-10 isolated from jeotgal, a traditional Korean fermented seafood

机译：从韩国传统发酵海鲜酒中分离出的芽孢杆菌SJ-10的高盐度条件下，耐盐性β-葡萄糖苷酶的热稳定性提高

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摘要

The beta-glucosidase gene, bglC, was cloned from Bacillus sp. SJ-10 isolated from the squid jeotgal. Recombinant BglC protein overexpression was induced in Escherichia coli. The optimal pH and temperature of the enzyme, using p-nitrophenyl-beta-d-glucopyranoside (pNP beta Glc) as a substrate, were pH 6 and 40 A degrees C, respectively. Enzymatic activity increased by 3.3- and 3.5-fold in the presence of 15 % NaCl and KCl, respectively. Furthermore, enzyme thermostability improved in the presence of NaCl or KCl. At 45 A degrees C in the presence of salts, the enzyme was stable for 2 h and maintained 80 % activity. In the absence of salts, BglC completely lost activity after 110 min at 45 A degrees C. Comparison of the kinetic parameters at various salt concentrations revealed that BglC had approximately 1.5- and 1.2-fold higher affinity and hydrolyzed pNP beta Glc 1.9- and 2.1-fold faster in the presence of 15 % NaCl and KCl, respectively. Additionally, the Gibb's free energy for denaturation was higher in the presence of 15 % salt than in the absence of salt at 45 and 50 A degrees C. Since enzymatic activity and thermostability were enhanced under high salinity conditions, BglC is an ideal salt-tolerant enzyme for further research and industrial applications.
机译：β-葡萄糖苷酶基因bglC从芽孢杆菌属（Bacillus sp。）从鱿鱼肠中分离出的SJ-10。在大肠杆菌中诱导重组BglC蛋白过表达。使用对硝基苯基-β-d-吡喃葡萄糖苷（pNPβGlc）作为底物的酶的最佳pH和温度分别为6和40 A摄氏度。在15％NaCl和KCl存在下，酶活性分别提高了3.3倍和3.5倍。此外，在NaCl或KCl存在下，酶的热稳定性得到改善。在盐存在下，在45 A的温度下，酶稳定2小时并保持80％的活性。在没有盐的情况下，BglC在45 A的温度下110分钟后完全失去活性。在各种盐浓度下的动力学参数比较显示，BglC的亲和力和水解的pNP beta Glc分别约为1.5和1.2倍，分别为1.9和2.1在分别含有15％NaCl和KCl的情况下，反应快两倍。此外，在15％的盐存在下，吉布的变性自由能比在45和50 A的温度下不存在盐的情况下更高。由于在高盐度条件下酶活性和热稳定性得到增强，BglC是理想的耐盐性酶用于进一步的研究和工业应用。

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