Objective To identify one Lactic Acid Bacteria isolated from Xinjiang traditional fermented camel milk in molecular biological level.Methods The strain was identified and analysed based on the data from DNA extraction,PCR amplification and 16S rDNA sequence analysis and phylogenetic analysis.Re-sults The rate of similarity of 16S rDNA sequencing in the strain with Lactobacillus pentosus (JCM 1558T ),Lactobacillus plantarum subsp .Argentoratensis (DKO 22 T ),Lactobacillus paraplantarum (DSM 10667T )and Lactobacillus plantarum subsp .Plantarum (ATCC 14917 T)reached 100%,99.87%, 99.8% and 99.74% after comparing with that in Eztaxon database,rpoA,conservative gene NCBI database for sequence alignment,sequence of lactic acid bacteria strains fermented in camel milk and reported lacto-bacillus strains 16 s rDNA,rpoA,pheS sequence using MEGA 5.2 software for phylogenetic analysis.Con-clusion The strains of 10 identified by molecular biological way is Lactobacillus pentosus (JCM 1558T).%目的：对新疆阿勒泰牧区哈萨克传统发酵驼乳中分离出的一株乳酸菌进行分子生物学鉴定。方法采用 DNA 提取、PCR 扩增、16S rDNA 产物测序和数据分析对该菌株进行系统发育分析鉴定。结果16S rD-NA 产物测序结果通过 Eztaxon 数据库比对，保守基因 rpoA、pheS 的扩增产物通过 NCBI 数据库比对，发酵驼乳中乳酸菌菌株的序列和已报道的乳酸菌菌株16S rDNA、rpoA、pheS 的序列分别用 MEGA 5．2软件进行系统发育分析表明：此菌株（10号）的16S rDNA 序列与 Lactobacillus pentosus （JCM 1558T ）、Lactobacillus plantarum subsp． Argentoratensis （DKO 22T ）、Lactobacillus paraplantarum （DSM 10667T ）和 Lactobacillus plantarum subsp． Plantarum （ATCC 14917T ）的相似率分别为100％、99．87％、99．8％和99．74％。结论10号乳酸菌菌种经分子生物学鉴定为 Lactobacillus pentosus （JCM 1558T ）。
展开▼
