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首页> 外文期刊>Bioprocess and Biosystems Engineering >Whole cell bioconversion of vitamin D-3 to calcitriol using Pseudonocardia sp KCTC 1029BP
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Whole cell bioconversion of vitamin D-3 to calcitriol using Pseudonocardia sp KCTC 1029BP

机译：使用假性心动过氧化物酶KCTC 1029BP将维生素D-3进行全细胞生物转化为骨化三醇

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摘要

Calcitriol is an important drug used for treating osteoporosis, which can be produced from vitamin D-3. The current method of producing calcitriol from vitamin D-3 during cultivation of microbial cells results in low yields of calcitriol and high purification costs. Therefore, in this study, the steps of cell cultivation and bioconversion of vitamin D-3 to calcitriol were separated. Cells of Pseudonocardia sp. KCTC 1029BP were utilized as a whole cell catalyst to produce a high level and yield of calcitriol from vitamin D-3. In addition, the effects of bioconversion buffers, cyclodextrins, and metal salts on the production of calcitriol were comparatively examined and selected for incorporation in the bioconversion medium, and their compositions were statistically optimized. The optimal bioconversion medium was determined as consisting of 15 mM Trizma base, 25 mM sodium succinate, 2 mM MgSO4, 0.08 % beta-cyclodextrin, 0.1 % NaCl, 0.2 % K2HPO4, and 0.03 % MnCl2. Using this optimal bioconversion medium, 61.87 mg/L of calcitriol, corresponding to a 30.94 % mass yield from vitamin D-3, was produced in a 75-L fermentor after 9 days. This calcitriol yield was 3.6 times higher than that obtained using a bioconversion medium lacking beta-cyclodextrin, NaCl, K2HPO4, and MnCl2. In conclusion, utilizing whole cells of Pseudonocardia sp. KCTC 1029BP together with the optimal bioconversion medium markedly enhanced the production of calcitriol from vitamin D-3.
机译：骨化三醇是用于治疗骨质疏松症的重要药物，其可以由维生素D-3产生。目前在微生物细胞培养过程中由维生素D-3生产骨化三醇的方法导致骨化三醇的收率低和纯化成本高。因此，在这项研究中，分离了细胞培养和维生素D-3转化为骨化三醇的步骤。假性心包菌的细胞KCTC 1029BP被用作全细胞催化剂，以从维生素D-3产生高含量和高产量的骨化三醇。此外，比较检查了生物转化缓冲液，环糊精和金属盐对骨化三醇的影响，并选择将其掺入生物转化培养基中，并对它们的组成进行了统计优化。确定最佳的生物转化培养基是由15 mM Trizma碱，25 mM琥珀酸钠，2 mM MgSO4、0.08％β-环糊精，0.1％NaCl，0.2％K2HPO4和0.03％MnCl2组成。使用这种最佳的生物转化培养基，在75天的发酵罐中放置9天后，产生了61.87 mg / L的骨化三醇，相当于维生素D-3的30.94％的质量产率。该骨化三醇的产率比使用缺乏β-环糊精，NaCl，K2HPO4和MnCl2的生物转化培养基获得的产率高3.6倍。总之，利用伪神经性心脏病的全细胞。 KCTC 1029BP与最佳生物转化培养基一起可显着提高维生素D-3生成骨化三醇的能力。

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