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首页> 外文期刊>Bioprocess and Biosystems Engineering >Ultrafiltration system for cyclodextrin production in repetitive batches by CGTase from Bacillus firmus strain 37
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Ultrafiltration system for cyclodextrin production in repetitive batches by CGTase from Bacillus firmus strain 37

机译：超滤系统，用于由坚定芽孢杆菌菌株37的CGTase重复生产批次的环糊精

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摘要

This study aimed to improve the yield of cyclodextrins (CDs) production in repetitive batches. An innovative ultrafiltration system was used to remove the inhibitory products that accumulated in the medium and to recover the enzyme. The assays were performed with the CGTase from Bacillus firmus strain 37 in purified, semi-purified, and crude extract forms. Maltodextrin (10 % w/v) and corn starch (5 % w/v) were used as substrates. After eight repetitive 24-h batches, the yield of beta-CD obtained with the purified enzyme and the corn starch substrate was 0.54 mmol/L/h, which was 36 % greater than that observed with the 10 % maltodextrin substrate. The crude CGTase extract with the corn starch substrate showed a productivity of 0.38 mmol/L/h, which was 29 % lower than using the purified enzyme and the corn starch substrate but 7 % higher than using the purified enzyme and the maltodextrin substrate. The crude extract, assayed with the corn starch substrate in the presence of 10 % ethanol reached 0.43 mmol/L/h productivity, which was 12 % higher compared to the assay without ethanol. The semi-purified enzyme was assayed with the corn starch substrate in the presence of 10 % ethanol for eight batches lasting 12 h and an excellent selectivity for the beta-CD was obtained, reaching a mean percentage of 96.0 %. Therefore, this ultrafiltration system enabled several batches of CD production, with efficient removal of products inhibitory to the CGTase and recovery of the enzyme. The possibility of industrial application of this system is promising.
机译：这项研究旨在提高重复批次中环糊精（CD）的产量。创新的超滤系统用于去除积聚在培养基中的抑制产物并回收酶。用来自纯化的，半纯化的和粗提取物形式的来自芽孢杆菌的菌株37的CGTase进行测定。麦芽糊精（10％w / v）和玉米淀粉（5％w / v）用作底物。在八次重复的24小时批处理后，用纯化的酶和玉米淀粉底物得到的β-CD的产量为0.54 mmol / L / h，比10％的麦芽糖糊精底物获得的产量高36％。具有玉米淀粉底物的粗制CGTase提取物的生产率为0.38 mmol / L / h，比使用纯化的酶和玉米淀粉底物低29％，但比使用纯化的酶和麦芽糊精底物高7％。在10％乙醇存在下用玉米淀粉底物测定的粗提物的生产率达到0.43 mmol / L / h，与不使用乙醇的测定相比提高了12％。在玉米淀粉底物存在10％的乙醇的情况下，使用八分批持续12小时的玉米淀粉底物进行了半纯化酶的分析，获得了极好的β-CD选择性，平均百分率达到96.0％。因此，这种超滤系统可实现几批CD的生产，并能有效去除CGTase抑制产物并回收该酶。该系统在工业上的应用前景广阔。

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