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首页> 外文期刊>Bioprocess and Biosystems Engineering >Production of polyunsaturated fatty acids in recombinant Lipomyces starkeyi through submerged fermentation
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Production of polyunsaturated fatty acids in recombinant Lipomyces starkeyi through submerged fermentation

机译：通过深层发酵在重组拟南芥中产生多不饱和脂肪酸

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Long chain polyunsaturated fatty acids (LCPUFA) are known to play an important role in human health and nutrition. Considering the limitation of LCPUFA sources, it is necessary to search new avenues for their production. Oleaginous yeasts are an attractive target for harvesting single cell oil, mainly because of the ease of cultivation with cheaper raw material. Lipomyces starkeyi is one such oleaginous yeast, which can accumulate oil to the extent of 60 % of its biomass and where genetic transformation can be achieved. In our earlier work, Delta 15 desaturase gene (AEP37840) from flax was transformed into L. starkeyi. In the present work, we report optimization of medium for the production of omega-3 enriched oil from this transformed yeast. A basic medium containing 20 g/l glucose as a carbon source and 10 g/l yeast extract as a nitrogen source was used during fermentation. At regular time intervals, glucose was fed to maintain high C:N ratio (65:10) during fermentation. Under the most favorable conditions, dry biomass and total lipid content were 18 and 7.29 g/l, respectively. Prior to genetic transformation, L. starkeyi contained 56.03 mg/l DHA along with 71.4 mg/l EPA and 42.2 mg/l ALA. Genetic engineering of this yeast resulted in a strain that produced 1080 mg/l DHA (17.4 %) along with 74.28 mg/l EPA and 126.72 mg/l ALA possibly through modification of PUFA biosynthetic pathway. To the best of our knowledge, this is a first report of DHA enrichment and opens up avenues for LCPUFA production through L. starkeyi.
机译：长链多不饱和脂肪酸（LCPUFA）在人类健康和营养中起着重要作用。考虑到LCPUFA来源的局限性，有必要寻找新的生产途径。含油酵母是收获单细胞油的一个有吸引力的目标，主要是因为使用较便宜的原料易于培养。 Starkeyi脂肪酵母是一种这样的油质酵母，可以将油积累至其生物量的60％，并且可以实现遗传转化。在我们早期的工作中，亚麻中的Delta 15去饱和酶基因（AEP37840）被转化为starkeyi L.。在目前的工作中，我们报告了从该转化酵母生产Omega-3浓缩油的培养基的优化。在发酵过程中，使用了含有20 g / l葡萄糖作为碳源和10 g / l酵母提取物作为氮源的基本培养基。在规则的时间间隔内，在发酵过程中喂入葡萄糖以维持高的C：N比（65:10）。在最有利的条件下，干生物量和总脂质含量分别为18和7.29 g / l。在遗传转化之前，starkeyi菌含有56.03 mg / l DHA以及71.4 mg / l EPA和42.2 mg / l ALA。对该酵母进行基因工程改造后，该菌株产生了1080 mg / l的DHA（17.4％）以及74.28 mg / l的EPA和126.72 mg / l的ALA，可能是通过修饰PUFA生物合成途径而产生的。就我们所知，这是DHA富集的第一份报告，并为通过L. starkeyi生产LCPUFA开辟了道路。

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