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首页> 外文期刊>European Journal of Lipid Science and Technology >Medium optimization for lipid production through co-fermentation of glucose and xylose by the oleaginous yeast Lipomyces starkeyi
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Medium optimization for lipid production through co-fermentation of glucose and xylose by the oleaginous yeast Lipomyces starkeyi

机译：产油酵母斯塔克霉菌共同发酵葡萄糖和木糖的脂质生产培养基优化

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摘要

Co-fermentation of lignocellulose-based carbohydrates is a potential solution to improve the economics of microbial lipid production. In the present paper, experiments were performed to optimize the media composition for lipid production by the oleaginous yeast Lipomyces starkeyi AS 2.1560 through co-fermentation of glucose and xylose (2 : 1 wt/wt). Statistical screening of nine media variables was performed by a Plackett-Burman design. Three factors, namely mixed sugar, yeast extract and FeSO4, were found as significant components influencing cellular lipid accumulation. Further optimization was carried out using a Box-Behnken factorial design to study the effects of these three variables on lipid production. A mathematical model with the R~2 value at 96.66% was developed to show the effect of each medium composition and their interactions on the lipid production. The model estimated that a maximal lipid content of 61.0 wt-% could be obtained when the concentrations of mixed sugar, yeast extract and FeSO4 were at 73.3 g/L (glucose 48.9 g/L, xylose 24.4 g/L), 7.9 g/L and 4.0 mg/L, respectively. The predicted value was in good accordance with the experimental data of 61.5%. Compared with the initial media, the optimized media gave 1.59-fold and 2.03-fold increases for lipid content and lipid productivity, respectively.
机译：基于木质纤维素的碳水化合物的共同发酵是提高微生物脂质生产经济性的潜在解决方案。在本文中，进行了实验，以通过葡萄糖和木糖（2：1 wt / wt）的共同发酵来优化油质酵母starkeyi AS 2.1560生产脂质的培养基组成。通过Plackett-Burman设计对9个媒体变量进行统计筛选。发现三个因素，即混合糖，酵母提取物和FeSO4，是影响细胞脂质积累的重要成分。使用Box-Behnken因子设计进行了进一步的优化，以研究这三个变量对脂质产生的影响。建立了R〜2值为96.66％的数学模型，以显示每种培养基组成及其相互作用对脂质产生的影响。该模型估计，当混合糖，酵母提取物和FeSO4的浓度分别为73.3 g / L（葡萄糖48.9 g / L，木糖24.4 g / L），7.9 g / L时，最大脂质含量为61.0 wt-％。 L和4.0 mg / L。预测值与61.5％的实验数据吻合良好。与初始培养基相比，优化培养基的脂质含量和脂质生产率分别提高了1.59倍和2.03倍。

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