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首页> 外文期刊>Bioprocess and Biosystems Engineering >Evaluation of different methods for immobilization of Candida antarctica lipase B (CalB lipase) in polyurethane foam and its application in the production of geranyl propionate
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Evaluation of different methods for immobilization of Candida antarctica lipase B (CalB lipase) in polyurethane foam and its application in the production of geranyl propionate

机译：评价在聚氨酯泡沫中固定南极假丝酵母脂肪酶B（CalB脂肪酶）的不同方法及其在丙酸香叶酯生产中的应用

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摘要

With the aim of studying the best method for the interaction of polyurethane (PU) foam and Candida antarctica lipase B, different methods of CalB immobilization were studied: adsorption (PU-ADS), bond (using polyethyleneimine) (PU-PEI), ionic adsorption by PEI with cross-linking with glutaraldehyde (PU-PEI-GA) and entrapment (PU). The characterization of immobilized enzyme derivatives was performed by apparent density and Fourier transform infrared spectroscopy. The free enzyme and enzyme preparations were evaluated at different pH values and temperatures. The highest enzyme activity was obtained using the PU method (5.52 U/g). The methods that stood out to compare the stabilities and kinetic parameters were the PU and PU-ADS. Conversions of 83.5 and 95.9 % for PU and PU-ADS derivatives were obtained, in 24 h reaction, using citronella oil and propionic acid as substrates.
机译：为了研究使聚氨酯泡沫与南极假丝酵母脂肪酶B相互作用的最佳方法，研究了CalB固定化的不同方法：吸附（PU-ADS），键合（使用聚乙烯亚胺）（PU-PEI），离子通过PEI进行吸附，并与戊二醛（PU-PEI-GA）交联并截留（PU）。固定化酶衍生物的表征是通过表观密度和傅里叶变换红外光谱进行的。在不同的pH值和温度下评估游离酶和酶制剂。使用PU方法获得最高的酶活性（5.52 U / g）。用来比较稳定性和动力学参数的突出方法是PU和PU-ADS。使用香茅油和丙酸作为底物，在24小时的反应中，PU和PU-ADS衍生物的转化率为83.5％和95.9％。

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