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首页> 外文期刊>Bioprocess and Biosystems Engineering >Biotransformation of lysine into cadaverine using barium alginate-immobilized Escherichia coli overexpressing CadA
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Biotransformation of lysine into cadaverine using barium alginate-immobilized Escherichia coli overexpressing CadA

机译:海藻酸钡固定化大肠杆菌过量表达CadA将赖氨酸生物转化为尸胺

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摘要

In this study, Escherichia coli cells overexpressing lysine decarboxylase (CadA) were used for cadaverine production. Barium alginate was selected as a matrix for immobilization of E. coli YH91. Free cells and immobilized cells (IC) were characterized for their physiochemical properties, and the optimum pH and temperature were determined as 6 and 37 A degrees C, respectively. Immobilized cells were three times more thermally stable compared to free cells at the optimum temperature and had a half-life (t (1/2)) of 131 h. The free cells lost most of lysine decarboxylase activity after nine cycles, but in contrast immobilized cells retained 56 % of their residual activity even after the 18th cycle. The immobilized cells gave a maximum production of cadaverine (75.8 g/L) with 84 % conversion.
机译:在这项研究中,过量表达赖氨酸脱羧酶(CadA)的大肠杆菌细胞被用于尸胺生产。选择海藻酸钡作为固定化大肠杆菌YH91的基质。对游离细胞和固定化细胞(IC)的理化特性进行了表征,最佳pH和温度分别确定为6和37 A摄氏度。在最佳温度下,固定化细胞的热稳定性是游离细胞的三倍,半衰期(t(1/2))为131小时。九个循环后,游离细胞丧失了大部分赖氨酸脱羧酶活性,但相比之下,固定化细胞甚至在第18个循环后仍保留了其剩余活性的56%。固定的细胞以84%的转化率产生了尸胺的最大产量(75.8 g / L)。

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