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Study of chemical pretreatment and enzymatic saccharification for producing fermentable sugars from rice straw

机译：化学预处理和酶促糖化制稻草发酵糖的研究

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This study evaluated a cost-effective approach for the conversion of rice straw into fermentable sugars. The composition of rice straw pretreated with 1 % sulfuric acid or 1 % sodium hydroxide solution was compared to rice straw with no chemical pretreatment. Enzymatic saccharification experiments on non-pretreated rice straw (NPRS), pretreated rice straw (PRS), and pretreated rice straw with acid hydrolysate (PRSAH) were conducted in a series of batch reactors. The results indicated that pre-treating the rice straw with dilute acid and base increased the cellulose content from 38 % to over 50 %. During enzymatic saccharification, straight aliphatic cellulose was hydrolyzed before branched hemicellulose, and glucose was the major hydrolysis product. The glucose yield was 0.52 g glucose/g for NPRS and was comparable to the yields of 0.50 g glucose/g for PRS and 0.58 g glucose/g for PRSAH. The hydrolysis of rice straw to produce glucose can be described by a first-order reaction with a rate constant of 0.0550 d~(-1) for NPRS, 0.0653 d~(-1) for PRSAH, and 0.0654 d~(-1) for PRS. Overall, the production of fermentable sugars from ground rice straw will be more cost effective if the straw is not pretreated with chemicals.
机译：这项研究评估了将稻草转化为可发酵糖的经济有效方法。将用1％硫酸或1％氢氧化钠溶液预处理过的稻草的组成与未经化学预处理的稻草进行了比较。在一系列间歇反应器中，对未经预处理的稻草（NPRS），经过预处理的稻草（PRS）和经过预处理的稻草与酸水解产物（PRSAH）进行了酶促糖化实验。结果表明，用稀酸和稀碱预处理稻草可以使纤维素含量从38％增加到50％以上。在酶促糖化过程中，直链脂族纤维素先水解成支链半纤维素，而葡萄糖是主要的水解产物。 NPRS的葡萄糖产量为0.52 g葡萄糖/ g，与PRS的0.50 g葡萄糖/ g和PRSAH的0.58 g葡萄糖/ g相当。稻草水解产生葡萄糖的过程可以通过一级反应来描述，速率常数对于NPRS为0.0550 d〜（-1），对于PRSAH为0.0653 d〜（-1），对于0.0654 d〜（-1）用于PRS。总体而言，如果稻草未经过化学预处理，则从稻谷秸秆生产可发酵糖的成本效益更高。

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