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首页> 外文期刊>Bioprocess and Biosystems Engineering >Optimization of exopolysaccharide production in submerged culture of Daedalea dickinsii and its antioxidant activity
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Optimization of exopolysaccharide production in submerged culture of Daedalea dickinsii and its antioxidant activity

机译：金钱草深层培养胞外多糖的优化及其抗氧化活性。

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摘要

The major nutrients, pH and temperature were evaluated for the exopolysaccharide (EPS) production by Daedalea dickinsii in submerged culture to derive an optimal medium composition and conditions as follows: 50 g/L maltose, 5 g/L soy peptone, 5 mM CaCl_2, at pH 6.0 and 28 ℃. A purified EPS fraction was attained from gel filtration chromatography and its major molecular characteristics were determined. FT-IR spectral analysis revealed the prominent characteristic groups of polyhydric alcohols. GC analysis and NMR spectrum showed its major molecular composition of glucose and galactose. Furthermore, thermogravimetric analysis indicated its degradation temperature (T_d) of 189 ℃. The antioxidant activity of the EPS fraction showed a correlation with the molecular properties. It might be attributed to the functional groups in the EPS fraction, which can donate electrons to reduce the radicals to a more stable form or react with the free radicals to terminate the radical chain reaction.
机译：通过淹没培养，评估了大叶ed（Daedalea dickinsii）产生的胞外多糖（EPS）的主要养分，pH和温度，得出了如下的最佳培养基组成和条件：50 g / L麦芽糖，5 g / L大豆蛋白ept，5 mM CaCl_2，在pH 6.0和28℃下。从凝胶过滤色谱获得纯化的EPS级分，并确定其主要分子特性。 FT-IR光谱分析揭示了多元醇的突出特征组。 GC分析和NMR谱显示其主要分子组成为葡萄糖和半乳糖。此外，热重分析表明其降解温度（T_d）为189℃。 EPS级分的抗氧化活性与分子性质相关。这可能归因于EPS部分中的官能团，该官能团可以提供电子以将自由基还原为更稳定的形式，或与自由基反应以终止自由基链反应。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2014年第7期|1401-1409|共9页
  • 作者

    Duo-Bin Mao; Chao-Wen Shi; Jian-Yong Wu; Chun-Ping Xu;

  • 作者单位

    College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450002, Henan, People's Republic of China;

    College of Life Science, Henan Agriculture University, Zhengzhou 450002, Henan, People's Republic of China;

    Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Kowloon, Hung Hom, Hong Kong, People's Republic of China;

    College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450002, Henan, People's Republic of China;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Daedalea dickinsii; Exopolysaccharides; Thermogravimetry; Antioxidants; NMR;

    机译：桃金娘;外多糖;热重法;抗氧化剂;核磁共振;

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