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首页> 外文期刊>Bioprocess and Biosystems Engineering >Production, characterization and antioxidant activity of exopolysaccharides from submerged culture of Morchella crassipes
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Production, characterization and antioxidant activity of exopolysaccharides from submerged culture of Morchella crassipes

机译：羊肚菌深层培养的胞外多糖的产生，表征和抗氧化活性

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摘要

The aim of this work was to investigate the fermentation optimization, molecular characterization, and antioxidant activity in vitro of exopolysaccharides (EPS) from Morchella crassipes in submerged culture. Firstly, an optimal medium for EPS production was obtained by single-factor experiment and central composite design as follows: maltose 44.79 g/L and tryptone 4.21 g/L. Then, one fraction of EPS was obtained from the culture filtrates by size exclusion chromatography and the molecular characteristics were examined by a multi-angle laser light scattering and refractive index detector system. The weight-average molar mass and the polydispersity ratio of the EPS fraction were revealed to be 1.961 × 10~4 g/mol and 1.838, respectively. FT-IR spectroscopy was used for obtaining vibrational spectra of the purified EPS fraction. Finally, the antioxidant activity of EPS was investigated and the relationship with molecular properties was discussed as well.
机译：这项工作的目的是调查深层培养的羊肚菌胞外多糖（EPS）的发酵优化，分子表征和体外抗氧化活性。首先，通过单因素实验和中心复合设计，获得了最佳的EPS产生培养基：麦芽糖44.79 g / L和胰蛋白4.2 4.21 g / L。然后，通过尺寸排阻色谱法从培养滤液中获得EPS的一部分，并通过多角度激光散射和折射率检测器系统检查分子特性。 EPS级分的重均摩尔质量和多分散度比分别为1.961×10〜4 g / mol和1.838。 FT-IR光谱用于获得纯化的EPS级分的振动光谱。最后，研究了EPS的抗氧化活性，并探讨了其与分子性能的关系。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2012年第8期|p.1325-1332|共8页
  • 作者

    Peixin He; Lujing Geng; Duobin Mao; Chunping Xu;

  • 作者单位

    College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450002, Henan,People's Republic of China;

    College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450002, Henan,People's Republic of China;

    College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450002, Henan,People's Republic of China;

    College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450002, Henan,People's Republic of China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    optimization; exopolysaccharides; morchella crassipes; antioxidant;

    机译：优化;胞外多糖;羊肚菌;抗氧化剂;

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