...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Bioprocess and Biosystems Engineering >Liquid perfluorochemical-supported hybrid cell culture system for proliferation of chondrocytes on fibrous polylactide scaffolds
【24h】

Liquid perfluorochemical-supported hybrid cell culture system for proliferation of chondrocytes on fibrous polylactide scaffolds

机译：液体全氟化物支持的混合细胞培养系统，用于在纤维聚丙交酯支架上增殖软骨细胞

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

CP5 bovine chondrocytes were cultured on biodegradable electrospun fibrous polylactide (PLA) scaffolds placed on a flexible interface formed between two immiscible liquid phases: (1) hydrophobic perfluorochem-ical (PFC) and (2) aqueous culture medium, as a new way of cartilage implant development. Robust and intensive growth of CP5 cells was achieved in our hybrid liquid-solid-liquid culture system consisting of the fibrous PLA scaffolds in contrast to limited growth of the CP5 cells in traditional culture system with PLA scaffold placed on solid surface. The multicellular aggregates of CP5 cells covered the surface of PLA scaffolds and the chondrocytes migrated through and overgrew internal fibers of the scaffolds. Our hybrid culture system simultaneously allows the adhesion of adherent CP5 cells to fibers of PLA scaffolds as well as, due to use of phase of PFC, enhances the mass transfer in the case of supplying/removing of respiratory gases, i.e., O_2 and CO_2. Our flexible (independent of vessel shape) system is simple, ready-to-use and may utilize a variety of polymer-based scaffolds traditionally proposed for implant development.
机译：CP5牛软骨细胞在可生物降解的电纺纤维聚丙交酯（PLA）支架上培养，该支架置于两个不混溶的液相之间形成的柔性界面上：（1）疏水性全氟化学（PFC）和（2）水性培养基，作为一种新的软骨方法植入物开发。在我们的由纤维PLA支架组成的液-固-液混合培养系统中，CP5细胞得以强劲且密集的生长，而在传统的培养系统中，将PLA支架放置在固体表面上，CP5细胞的生长有限。 CP5细胞的多细胞聚集体覆盖了PLA支架的表面，软骨细胞迁移通过并使其生长过度。我们的混合培养系统同时允许粘附的CP5细胞粘附到PLA支架的纤维上，并且由于使用了PFC相，在供应/除去呼吸气体（即O_2和CO_2）的情况下，可以增强质量传递。我们灵活的（与血管形状无关）系统简单易用，并且可以利用传统上建议用于植入物开发的多种基于聚合物的支架。

著录项

  • 来源
    《Bioprocess and Biosystems Engineering》 |2014年第9期|1707-1715|共9页
  • 作者

    Maciej Pilarek; Iwona Grabowska; Ilona Senderek; Michal Wojasinski; Justyna Janicka; Katarzyna Janczyk-Ilach; Tomasz Ciach;

  • 作者单位

    Biotechnology and Bioprocess Engineering Division, Faculty of Chemical and Process Engineering, Warsaw University of Technology, Warynskiego 1, 00-645 Warsaw, Poland;

    Department of Cytology, Institute of Zoology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland;

    Biotechnology and Bioprocess Engineering Division, Faculty of Chemical and Process Engineering, Warsaw University of Technology, Warynskiego 1, 00-645 Warsaw, Poland;

    Biotechnology and Bioprocess Engineering Division, Faculty of Chemical and Process Engineering, Warsaw University of Technology, Warynskiego 1, 00-645 Warsaw, Poland;

    Biotechnology and Bioprocess Engineering Division, Faculty of Chemical and Process Engineering, Warsaw University of Technology, Warynskiego 1, 00-645 Warsaw, Poland;

    Department of Cytology, Institute of Zoology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland;

    Biotechnology and Bioprocess Engineering Division, Faculty of Chemical and Process Engineering, Warsaw University of Technology, Warynskiego 1, 00-645 Warsaw, Poland;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Liquid/liquid culture system; Perfluorochemical (fluorocarbon); Polylactide (PLA) Fibrous scaffold; Electrospinning; Chondrocytes;

    机译：液/液培养系统;全氟化物（碳氟化合物）;聚乳酸（PLA）纤维支架;电纺;软骨细胞;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号