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首页> 外文期刊>Bioprocess and Biosystems Engineering >Screening of phenylpyruvic acid producers and optimization of culture conditions in bench scale bioreactors
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Screening of phenylpyruvic acid producers and optimization of culture conditions in bench scale bioreactors

机译：苯丙酮酸生产者的筛选和台式生物反应器中培养条件的优化

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摘要

Alpha keto acids are deaminated forms of amino acids that have received significant attention as feed and food additives in the agriculture and medical industries. To date, their production has been commonly performed at shake-flask scale with low product concentrations. In this study, production of phenylpyruvic acid (PPA), which is the alpha keto acid of phenylalanine was investigated. First, various microorganisms were screened to select the most efficient producer. Thereafter, growth parameters (temperature, pH, and aeration) were optimized in bench scale bioreactors to maximize both PPA and biomass concentration in bench scale bioreactors, using response surface methodology. Among the four different microorganisms evaluated, Proteus vulgaris was the most productive strain for PPA production. Optimum temperature, pH, and aeration conditions were determined as 34.5 ℃, 5.12, and 0.5 vvm for PPA production, whereas 36.9 ℃, pH 6.87, and 0.96 vvm for the biomass production. Under these optimum conditions, PPA concentration was enhanced to 1,054 mg/L, which was almost three times higher than shake-flask fermentation concentrations. Moreover, P. vulgaris biomass was produced at 3.25 g/L under optimum conditions. Overall, this study demonstrated that optimization of growth parameters improved PPA production in 1-L working volume bench-scale bioreactors compared to previous studies in the literature and was a first step to scale up the production to industrial production.
机译：α-酮酸是氨基酸的脱氨基形式，在农业和医疗行业中作为饲料和食品添加剂受到了广泛关注。迄今为止，它们的生产通常是在摇瓶规模下以低产物浓度进行的。在这项研究中，研究了苯丙酮酸（PPA）的生产，苯丙酮酸是苯丙氨酸的α-酮酸。首先，筛选各种微生物以选择最有效的生产者。此后，使用响应表面方法，在台式生物反应器中优化了生长参数（温度，pH和曝气），以最大化台式生物反应器中的PPA和生物质浓度。在评估的四种不同微生物中，寻常变形杆菌是生产PPA的最有生产力的菌株。确定PPA生产的最佳温度，pH和通气条件分别为34.5℃，5.12和0.5 vvm，而生物质生产的最佳温度，pH和通气条件确定为36.9℃，pH 6.87和0.96 vvm。在这些最佳条件下，PPA浓度增加到1,054 mg / L，几乎是摇瓶发酵浓度的三倍。此外，在最佳条件下，寻常型腐生菌的生物量为3.25 g / L。总的来说，这项研究表明，与文献中先前的研究相比，优化生长参数可以提高1-L工作体积台式生物反应器的PPA产量，这是将生产规模扩大到工业生产的第一步。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2014年第11期|2343-2352|共10页
  • 作者

    Hasan B. Coban; Ali Demirci; Paul H. Patterson; Ryan J. Elias;

  • 作者单位

    Department of Agricultural and Biological Engineering, The Pennsylvania State University, University Park, PA 16802, USA;

    Department of Agricultural and Biological Engineering, The Pennsylvania State University, University Park, PA 16802, USA ,The Huck Institutes of Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA;

    Department of Animal Science, The Pennsylvania State University, University Park, PA 16802, USA;

    Department of Food Science, The Pennsylvania State University, University Park, PA 16802, USA;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Phenylpyruvic acid; Proteus vulgaris; Optimization growth parameters; Submerged fermentation;

    机译：苯丙酮酸;寻常变形杆菌;优化生长参数;浸没式发酵;

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