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首页> 外文期刊>Turkish journal of biology >Optimization of immobilization conditions by conventional and statistical strategies for alkaline lipase production by Pseudomonas aeruginosa mutant cells: Scale-up at bench-scale bioreactor level
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Optimization of immobilization conditions by conventional and statistical strategies for alkaline lipase production by Pseudomonas aeruginosa mutant cells: Scale-up at bench-scale bioreactor level

机译：通过常规和统计策略优化铜绿假单胞菌突变体细胞产生碱性脂肪酶的固定条件：在台式生物反应器水平上扩大规模

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摘要

Suitability of 3 matrices, agarose, sodium alginate, and polyacrylamide, for immobilization of mutant cells of Pseudomonas aeruginosa MTCC 10,055 was investigated. Of these, agarose was proven to be the best as exhibiting maximum enzyme production (4363.4 U/mL), followed by polyacrylamide gel (2172.3 U/mL). Alginate beads were the poorest. The one-variable-at-a-time approach suggested agarose at 2.0%, immobilized bead at 4.0 g blocks/50 mL, and initial cell loading of 0.8 g in the matrix as optimum conditions for maximum lipase production (5982.3 U/mL) after 24 h of incubation. However, response surface methodology studies determined the optimum values of these variables as 1.96%, 4.06 g blocks/50 mL, and 0.81 g of cells in the matrix for maximum lipase production (6354.23 U/mL) within 22.54 h of incubation. The agarose blocks were reusable for 7 cycles without any significant loss in lipase yield. Bench-scale bioreactor level optimization resulted in further enhancement in lipase yield (6815.3 U/mL) at 0.6 vvm aeration and 100 rpm agitation within only 20 h of incubation. Presumably, this is the first attempt for lipase production by immobilized cells of P. aeruginosa at the bioreactor level. The agarose-immobilized mutant cells showed potential candidature for alkaline lipase production at the industrial level.
机译：研究了3种基质（琼脂糖，藻酸钠和聚丙烯酰胺）对铜绿假单胞菌MTCC 10,055突变细胞的固定性。其中，琼脂糖被证明是最好的，表现出最大的酶产生（4363.4 U / mL），其次是聚丙烯酰胺凝胶（2172.3 U / mL）。藻酸盐珠是最贫穷的。一次可变的方法建议使用2.0％的琼脂糖，4.0 g块/ 50 mL的固定化珠子和0.8 g的初始细胞上样量作为最大脂肪酶产量的最佳条件（5982.3 U / mL）孵育24小时后。但是，响应面方法学研究确定了这些变量的最佳值，分别为1.96％，4.06 g块/ 50 mL和0.81 g基质中的细胞，可在孵育22.54小时内获得最大的脂肪酶产量（6354.23 U / mL）。琼脂糖块可重复使用7个循环，而脂肪酶的产量没有任何明显的损失。台式规模的生物反应器水平优化可在仅孵育20小时的0.6 vvm曝气和100 rpm搅拌下进一步提高脂肪酶的产量（6815.3 U / mL）。据推测，这是固定化铜绿假单胞菌细胞在生物反应器水平生产脂肪酶的首次尝试。固定在琼脂糖上的突变细胞在工业水平上显示出潜在的碱性脂肪酶生产候选资格。

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