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首页> 外文期刊>Bioprocess and Biosystems Engineering >Lipase from marine strain using cooked sunflower oil waste: production optimization and application for hydrolysis and thermodynamic studies
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Lipase from marine strain using cooked sunflower oil waste: production optimization and application for hydrolysis and thermodynamic studies

机译：使用煮熟的葵花籽油废料从海洋菌株中提取脂肪酶：生产优化以及在水解和热力学研究中的应用

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摘要

The marine strain Pseudomonas otitidis was isolated to hydrolyze the cooked sunflower oil (CSO) followed by the production of lipase. The optimum culture conditions for the maximum lipase production were determined using Plackett-Burman design and response surface methodology. The maximum lipase production, 1,980 U/ml was achieved at the optimum culture conditions. After purification, an 8.4-fold purity of lipase with specific activity of 5,647 U/mg protein and molecular mass of 39 kDa was obtained. The purified lipase was stable at pH 5.0-9.0 and temperature 30-80 ℃. Ca~(2+) and Triton X-100 showed stimulatory effect on the lipase activity. The purified lipase was highly stable in the non-polar solvents. The functional groups of the lipase were determined by Fourier transform-infrared (FT-IR) spectroscopy. The purified lipase showed higher hydrolytic activity towards CSO over the other cooked oil wastes. About 92.3 % of the CSO hydrolysis was observed by the lipase at the optimum time 3 h, pH 7.5 and temperature 35 ℃. The hydrolysis of CSO obeyed pseudo first order rate kinetic model. The thermodynamic properties of the lipase hydrolysis were studied using the classical Van't Hoff equation. The hydrolysis of CSO was confirmed by FT-IR studies.
机译：分离出海洋菌株奥氏假单胞菌以水解煮熟的葵花油（CSO），然后产生脂肪酶。使用Plackett-Burman设计和响应面方法确定最大脂肪酶产生的最佳培养条件。在最佳培养条件下，最大脂肪酶产量为1,980 U / ml。纯化后，获得的脂肪酶纯度为8.4倍，比活为5647 U / mg蛋白，分子量为39 kDa。纯化的脂肪酶在pH 5.0-9.0和温度30-80℃下稳定。 Ca〜（2+）和Triton X-100对脂肪酶活性有刺激作用。纯化的脂肪酶在非极性溶剂中高度稳定。脂肪酶的官能团通过傅里叶变换红外（FT-IR）光谱法确定。纯化的脂肪酶显示出比其他熟油废料更高的对CSO的水解活性。在最佳时间3 h，pH 7.5和温度35℃下，脂肪酶观察到约92.3％的CSO水解。 CSO的水解遵循伪一级速率动力学模型。使用经典的Van't Hoff方程研究了脂肪酶水解的热力学性质。通过FT-IR研究证实了CSO的水解。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2013年第3期|301-315|共15页
  • 作者

    K. Ramani; P. Saranya; S. Chandan Jain; G. Sekaran;

  • 作者单位

    Environmental Technology Division, Council of Scientific and Industrial Research (CSIR), Central Leather Research Institute (CLRI), Adyar, Chennai 600 020, India;

    Environmental Technology Division, Council of Scientific and Industrial Research (CSIR), Central Leather Research Institute (CLRI), Adyar, Chennai 600 020, India;

    Environmental Technology Division, Council of Scientific and Industrial Research (CSIR), Central Leather Research Institute (CLRI), Adyar, Chennai 600 020, India;

    Environmental Technology Division, Council of Scientific and Industrial Research (CSIR), Central Leather Research Institute (CLRI), Adyar, Chennai 600 020, India;

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  • 正文语种 eng
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  • 关键词

    marine pseudomonas otitidis; cooked sunflower oil; lipase; hydrolysis; rate kinetic models; thermodynamic studies;

    机译：海洋假单胞菌煮熟的葵花籽油;脂肪酶水解;速率动力学模型热力学研究;

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