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首页> 外文期刊>Bioprocess and Biosystems Engineering >A new genotype of Miscanthus sacchariflorus Geodae-Uksae 1,identified by growth characteristics and a specific SCAR marker
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A new genotype of Miscanthus sacchariflorus Geodae-Uksae 1,identified by growth characteristics and a specific SCAR marker

机译：通过生长特性和特定的SCAR标记鉴定出一种新的基因型的芒草（Miscanthus sacchariflorus Geodae-Uksae）1

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摘要

Miscanthus is referred to as an ideal lignocel-lulosic bioenergy crop, which can be used to generate heat, power, and fuel, as well as to reduce carbon dioxide emissions. The new Miscanthus sacchariflorus genotype named Geodae-Uksae 1 was recently collected from damp land in southern Korea. This study investigated the growth characteristics of Miscanthus genotypes, and developed a specific, sensitive, and reproducible sequence characterized amplified region (SCAR) marker to distinguish new M. sacchariflorus genotype Geodae-Uksae 1 from other native Miscanthus species in Korea. Growth characteristics such as stem length, stem diameter, and dry weight of Geodae-Uksae 1 were greater than those of normal M. sacchariflorus. The genotypes within Geodae-Uksae 1 were had the highest genetic similarity. A putative 1,800-bp polymorphic sequence specific to Geodae-Uksae 1 was identified with the random amplified polymorphic DNA (RAPD) N8018 primer. The sequence-characterized amplified region (SCAR) primers Geodae 1-F and Geodae 1-R were designed based on the unique RAPD amplicon. The SCAR primers produced a specific 1,799-bp amplicon in authentic Geodae-Uksae 1, whereas no amplification was observed in other Miscanthus species. The SCAR marker could contribute to identify Geodae-Uksae 1 among native Miscanthus species. The new Miscanthus genotype Geodae-Uksae 1 has great potential as an alternative lignocellulosic bio-mass feedstock for bioenergy productions.
机译：芒草被称为一种理想的木质纤维素生物能源作物，可用于产生热量，电力和燃料，并减少二氧化碳的排放。最近从韩国南部的潮湿土地上收集到了一种新的基因型，名为Geodae-Uksae 1的糖蔗糖基因。这项研究调查了芒草基因型的生长特征，并开发了一种特异性，灵敏和可再现的序列特征扩增区域（SCAR）标记，以区分新的糖荚果分支杆菌基因型Geodae-Uksae 1与韩国其他本土芒草种。 Geodae-Uksae 1的茎长，茎径和干重等生长特性均比正常糖蔗分枝杆菌更大。 Geodae-Uksae 1内的基因型具有最高的遗传相似性。利用随机扩增的多态性DNA（RAPD）N8018引物鉴定出了对Geodae-Uksae 1特异的假定的1,800-bp多态性序列。基于独特的RAPD扩增子设计了序列特征扩增区（SCAR）引物Geodae 1-F和Geodae 1-R。 SCAR引物在真实的Geodae-Uksae 1中产生了一个特定的1,799-bp扩增子，而在其他芒草中未观察到扩增。 SCAR标记可能有助于在本地芒草物种中鉴定Geodae-Uksae 1。新的芒草基因型Geodae-Uksae 1具有巨大的潜力，可以作为替代木质纤维素生物质原料用于生产生物能源。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2013年第6期|695-703|共9页
  • 作者

    Gi Hong An; Jung Kon Kim; Youn-Ho Moon; Young-Lok Cha; Young Mi Yoon; Bon-Cheol Koo; Kwang-Guen Park;

  • 作者单位

    Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration, Muan, Chonnam 534-833, South Korea;

    National Institute of Animal Science, Rural Development Administration, 77 Chuksan gil, Gwonsun-Gu,Suwon, South Korea;

    Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration, Muan, Chonnam 534-833, South Korea;

    Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration, Muan, Chonnam 534-833, South Korea;

    Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration, Muan, Chonnam 534-833, South Korea;

    Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration, Muan, Chonnam 534-833, South Korea;

    Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration, Muan, Chonnam 534-833, South Korea;

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  • 关键词

    Bioenergy; Geodae-Uksae 1; Lignocellulosic biomass feedstock; Miscanthus; RAPD-PCR; SCAR marker;

    机译：生物能源Geodae-Uksae 1;木质纤维素生物质原料;芒草;RAPD-PCR;SCAR标记;

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