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首页> 外文期刊>Bioprocess and Biosystems Engineering >Development of SCAR marker for simultaneous identification of Miscanthus sacchariflorus, M. sinensis and M. x giganteus
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Development of SCAR marker for simultaneous identification of Miscanthus sacchariflorus, M. sinensis and M. x giganteus

机译：SCAR标记的开发，可同时鉴定糖芒，中华芒和巨型芒

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摘要

The sequence-characterized amplified region (SCAR) marker for simultaneous identification of Miscanthus sacchariflorus, Miscanthus sinensis, and Miscanthus x giganteus was developed. In this study, it was attempted for the first time to develop the SCAR marker for detecting the molecular phenotypes among Miscanthus species. Randomly amplified polymorphic DNA technique was applied for this study and one fragment which is unique to M. sacchariflorus was identified and then sequenced. Based on the specific fragment, one SCAR primer pair designated as MS62-5F and MS62-5R was designed to amplify an approximately 1,000 bp DNA fragment within the sequenced region. Diagnostic PCR was performed using the primer pair. Using this SCAR marker, approximately 1,000 bp and 1,200 bp DNA fragments were obtained in M. sacchariflorus and M. sinensis, respectively. Moreover, M. x giganteus was obtained both bands at the same time. The result showed that this SCAR marker can clearly distinguish the M. sacchariflorus, M. sinensis, and M. x giganteus, respectively.
机译：开发了序列特征扩增区（SCAR）标记，用于同时鉴定糖芒草，芒草和芒草x giganteus。在这项研究中，首次尝试开发SCAR标记来检测芒草物种的分子表型。这项研究应用了随机扩增的多态性DNA技术，并鉴定了一个糖精分枝杆菌特有的片段，然后进行了测序。根据特定片段，设计了一个名为MS62-5F和MS62-5R的SCAR引物对，以扩增测序区域内约1,000 bp的DNA片段。使用引物对进行诊断PCR。使用该SCAR标记，在糖藻分枝杆菌和中华分枝杆菌中分别获得了大约1,000 bp和1,200 bp的DNA片段。此外，M。x giganteus是同时获得的两个波段。结果表明，该SCAR标记可以清楚地区分M. sacchariflorus，M。sinensis和M. x giganteus。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2012年第2期|p.55-59|共5页
  • 作者

    Jung Kon Kim; Gi Hong An; Seung-Hyun Ahn; Youn-Ho Moon; Young-Lok Cha; Surn-Teh Bark; Yong-Hwan Choi; Sae-Jung Suh; Sang-Gyu Seo; Sun-Hyung Kim; Bon-Cheol Koo;

  • 作者单位

    Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration, 293-5,Cheongcheon, Cheonggye, Muan, Jeonnam 534-833,South Korea;

    Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration, 293-5,Cheongcheon, Cheonggye, Muan, Jeonnam 534-833,South Korea;

    Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration, 293-5,Cheongcheon, Cheonggye, Muan, Jeonnam 534-833,South Korea;

    Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration, 293-5,Cheongcheon, Cheonggye, Muan, Jeonnam 534-833,South Korea;

    Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration, 293-5,Cheongcheon, Cheonggye, Muan, Jeonnam 534-833,South Korea;

    Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration, 293-5,Cheongcheon, Cheonggye, Muan, Jeonnam 534-833,South Korea;

    Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration, 293-5,Cheongcheon, Cheonggye, Muan, Jeonnam 534-833,South Korea;

    Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration, 293-5,Cheongcheon, Cheonggye, Muan, Jeonnam 534-833,South Korea;

    Department of Environmental Horticulture, The University of Seoul, 90, Jeonnong-dong,Dongdaemun-gu, Seoul 130-743, South Korea;

    Department of Environmental Horticulture, The University of Seoul, 90, Jeonnong-dong,Dongdaemun-gu, Seoul 130-743, South Korea;

    Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration, 293-5,Cheongcheon, Cheonggye, Muan, Jeonnam 534-833,South Korea;

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  • 正文语种 eng
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  • 关键词

    miscanthus; SCAR marker; RAPD; bioenergy; molecular phenotype;

    机译：can SCAR标记;RAPD;生物能源分子表型;

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