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首页> 外文期刊>Bioprocess and Biosystems Engineering >Optimization of lipase-catalyzed synthesis of caffeic acid phenethyl ester in ionic liquids by response surface methodology
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Optimization of lipase-catalyzed synthesis of caffeic acid phenethyl ester in ionic liquids by response surface methodology

机译：响应面法优化脂肪酶催化离子液体中咖啡酸苯乙酯的合成

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Lipase-catalyzed caffeic acid phenethyl ester (CAPE) synthesis in ionic liquid, l-ethyl-3-methylimida-zolium bis[(trifluoromethyl)sulfonyl]imide ([Emim] [Tf_2N]), was investigated in this study. The effects of several reaction conditions, including reaction time, reaction temperature, substrate molar ratio of phenethyl alcohol to caffeic acid (CA), and weight ratio of enzyme to CA, on CAPE yield were examined. In a single parameter study, the highest CAPE yield in [Emim][Tf_2N] was obtained at 70 ℃ with a substrate molar ratio of 30:1 and weight ratio of enzyme to CA of 15:1. Based on these results, response surface methodology (RSM) with a 3-level-4-factor central composite rotatable design (CCRD) was adopted to evaluate enzymatic synthesis of CAPE in [Emim][Tf_2N]. The four major factors were reaction time (36-60 h), reaction temperature (65-75 ℃), substrate molar ratio of phenethyl alcohol to CA (20:1 -10:1), and weight ratio of enzyme to CA (10:1 -20:1). A quadratic equation model was used to analyze the experimental data at a 95 % confidence level (p < 0.05). A maximum conversion yield of 99.8 % was obtained under the optimized reaction conditions [60 h, 73.7 ℃, substrate molar ratio of phenethyl alcohol to CA (27.1:1), and weight ratio of enzyme to CA (17.8:1)] established by our statistical method, whereas the experimental conversion yield was 96.6 ± 2 %.
机译：本研究研究了在离子液体中的脂肪酶催化的咖啡酸苯乙酯（CAPE）的合成，即1-乙基-3-甲基亚咪唑鎓双[（三氟甲基）磺酰基]酰亚胺（[Emim] [Tf_2N]）。研究了几种反应条件，包括反应时间，反应温度，苯乙醇与咖啡酸的底物摩尔比（CA）以及酶与CA的重量比对CAPE收率的影响。在单参数研究中，[Emim] [Tf_2N]的最高CAPE产量在70℃下获得，底物摩尔比为30：1，酶与CA的重量比为15：1。基于这些结果，采用具有3级4要素中心复合可旋转设计（CCRD）的响应面方法（RSM）评估[Emim] [Tf_2N]中CAPE的酶促合成。四个主要因素是反应时间（36-60 h），反应温度（65-75℃），苯乙醇与CA的底物摩尔比（20：1 -10：1）和酶与CA的重量比（10 ：1 -20：1）。使用二次方程模型分析置信水平为95％（p <0.05）的实验数据。在最佳反应条件下[60h，73.7℃，苯乙醇与CA的底物摩尔比（27.1：1），酶与CA的重量比（17.8：1）]获得最高转化率99.8％。我们的统计方法，而实验转化率为96.6±2％。

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