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首页> 外文期刊>Bioprocess and Biosystems Engineering >Reaction engineering analysis of cellulase production with Trichoderma reesei RUT-C30 with intermittent substrate supply
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Reaction engineering analysis of cellulase production with Trichoderma reesei RUT-C30 with intermittent substrate supply

机译：带有间歇底物供应的里氏木霉RUT-C30生产纤维素酶的反应工程分析

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摘要

The effects of varying initial concentrations of microcrystalline cellulose on cellulase production with Trichoderma reesei RUT-C30 as well as the effects of varying lactose and ammonium sulfate concentrations in the feed medium were studied simultaneously in parallel-operated shake flasks and, alternatively, in parallel-operated stirred-tank bioreactors on a 10-mL scale. Fifteen experiments were performed as triplicates in shake flasks as well as in stirred-tank bioreactors in parallel to identify the parameters of second-order polynomials for the estimation of the final filter paper activity of T. reesei RUT-C30 after a process time of 96 h. Even though parameter estimation was not possible based on the results of the shake flasks due to final enzyme activities at or below the detection limit (with the exception of one shake flask), the identification of the second-order polynomial was successful with the results of the parallel-operated stirred-tank bioreactors on a 10-mL scale. Reaction conditions with 53.3 gL~(-1) microcrystalline cellulose in the initial medium, no lactose feeding and 3.3 g L~(-1) day~(-1) intermittent ammonium sulfate addition were estimated to be optimal. The final experimental validation of the optimum substrate supply on a L-scale resulted in the production of 4.88 filter paper units (FPU) mL~(-1) with T. reesei RUT-C30 after 96 h. This is an improvement by a factor of 3.6 compared to the reference batch process (1.35 FPU mL~(-1)).
机译：在平行操作的摇瓶中同时研究了不同初始浓度的微晶纤维素对里氏木霉RUT-C30纤维素酶生产的影响以及进料培养基中乳糖和硫酸铵浓度变化的影响，操作10毫升规模的搅拌罐式生物反应器。在摇瓶中以及在搅拌罐式生物反应器中平行进行15个实验，一式三份，以鉴定二阶多项式的参数，以估计在96个处理时间后里氏木霉RUT-C30的最终滤纸活性H。即使由于最终酶活度达到或低于检测极限（一个摇瓶除外）而无法根据摇瓶的结果进行参数估计（通过一个摇瓶除外），仍可以成功地识别出二阶多项式平行操作的搅拌槽生物反应器的体积为10毫升。在初始培养基中，以53.3 gL〜（-1）微晶纤维素为原料，不加乳糖，以3.3 g L〜（-1）day〜（-1）间歇添加硫酸铵的反应条件为最佳。在L规模上对最佳基材供应进行了最终实验验证，结果是在96小时后用里氏木霉RUT-C30生产了4.88个滤纸单元（FPU）mL〜（-1）。与参考批处理（1.35 FPU mL〜（-1））相比，这提高了3.6倍。

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