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首页> 外文期刊>Bioprocess and Biosystems Engineering >Citric acid production from extract of Jerusalem artichoke tubers by the genetically engineered yeast Yarrowia lipolytica strain 30 and purification of citric acid
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Citric acid production from extract of Jerusalem artichoke tubers by the genetically engineered yeast Yarrowia lipolytica strain 30 and purification of citric acid

机译：转基因酵母解脂耶氏酵母菌株30从菊芋提取物中提取柠檬酸并纯化柠檬酸

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摘要

In this study, citric acid production from extract of Jerusalem artichoke tubers by the genetically engineered yeast Yarrowia lipolytica strain 30 was investigated. After the compositions of the extract of Jerusalem artichoke tubers for citric acid production were optimized, the results showed that natural components of extract of Jerusalem artichoke tubers without addition of any other components were suitable for citric acid production by the yeast strain. During 10 L fermentation using the extract containing 84.3 g L~(-1) total sugars, 68.3 g L~(-1) citric acid was produced and the yield of citric acid was 0.91 g g~(-1) within 336 h. At the end of the fermentation, 9.2 g L~(-1) of residual total sugar and 2.1 g L~(-1) of reducing sugar were left in the fermented medium. At the same time, citric acid in the supernatant of the culture was purified. It was found that 67.2 % of the citric acid in the supernatant of the culture was recovered and purity of citric acid in the crystal was 96%.
机译：在这项研究中，研究了转基因酵母解脂耶氏酵母菌株30从菊芋提取物中的柠檬酸生产。对菊芋生产柠檬酸的提取物的成分进行优化后，结果表明，不添加任何其他成分的菊芋提取物的天然成分适用于酵母菌株生产柠檬酸。在使用含有84.3 g L〜（-1）总糖的提取物进行10 L发酵的过程中，产生了68.3 g L〜（-1）柠檬酸，在336小时内柠檬酸的产量为0.91 g g〜（-1）。发酵结束时，发酵培养基中残留了9.2 g L〜（-1）残留糖和2.1 g L〜（-1）还原糖。同时，纯化培养物上清液中的柠檬酸。发现在培养物的上清液中回收了67.2％的柠檬酸，并且晶体中柠檬酸的纯度为96％。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2013年第11期|1759-1766|共8页
  • 作者

    Ling-Fei Wang; Zhi-Peng Wang; Xiao-Yan Liu; Zhen-Ming Chi;

  • 作者单位

    Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, Shandong 266003, China;

    Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, Shandong 266003, China;

    Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, Shandong 266003, China;

    Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, Shandong 266003, China;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Citric acid; Engineered Yarrowia lipolytica; Inulin; Crystal; Jerusalem artichoke tubers;

    机译：柠檬酸;工程化解脂耶氏酵母;菊粉;水晶;菊芋块茎;

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