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Polyunsaturated fatty acid production by marine bacteria

机译：海洋细菌生产多不饱和脂肪酸

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摘要

Polyunsaturated fatty acids are important in maintaining human health. Limitations associated with current sources of ω-3 fatty acids and ω-6 fatty acids, from animal and plant sources, have led to increased interest in microbial production. Marine bacteria may provide a suitable alternative, although the isolation of production strains and the identification of operating conditions must be addressed before manufacturing processes become economically viable. Marine isolate 560 was identified as an eicosapentaenoic acid (EPA) producer via GC/MS. The isolate was initially identified as Vibrio cyclitrophicus by 16S rRNA sequencing. Statistically based experimental designs were applied to the optimisation of medium components and environmental factors for the production of EPA. A Plackett-Burman design was used to screen for the effect of temperature, pH, and media components. Subsequently, the concentrations of NaCl, yeast extract, and peptone, identified as significant factors, were optimised using a central composite design. The predicted optimal combination of media components for maximum EPA production (4.8 mg/g dry weight) was determined as 7.9 g/l peptone, 16.2 g/l NaCl, and 6.2 g/l yeast extract. On transfer of this process to bioreactor cultivation, where a range of pH and DO values were tested, the maximum amount of EPA produced increased to 7.5 mg/g dry weight and 10 % of the total fatty acid.
机译：多不饱和脂肪酸对维持人类健康很重要。与动植物来源的现有ω-3脂肪酸和ω-6脂肪酸来源相关的局限性导致人们对微生物生产的兴趣增加。尽管在生产过程变得经济可行之前必须解决生产菌株的分离和操作条件的确定，但海洋细菌可能提供了合适的替代方法。通过GC / MS将海洋分离物560鉴定为二十碳五烯酸（EPA）的生产者。该分离物最初通过16S rRNA测序鉴定为弧菌弧菌。基于统计的实验设计被应用于培养基成分和环境因素的优化，以生产EPA。使用Plackett-Burman设计来筛选温度，pH和介质成分的影响。随后，使用中央复合设计优化确定为重要因素的NaCl，酵母提取物和蛋白ept的浓度。确定最大EPA产量（4.8 mg / g干重）的培养基组分的预测最佳组合为7.9 g / l蛋白ept，16.2 g / l NaCl和6.2 g / l酵母提取物。在将该过程转移到生物反应器培养中后，在其中测试了一系列的pH和DO值，所产生的EPA的最大量增加到7.5 mg / g干重和10％的总脂肪酸。

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