...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Bioprocess and Biosystems Engineering >Accelerated production of 1,3-propanediol from glycerol by Klebsiella pneumoniae using the method of forced pH fluctuations
【24h】

Accelerated production of 1,3-propanediol from glycerol by Klebsiella pneumoniae using the method of forced pH fluctuations

机译：使用强制性pH波动方法可通过肺炎克雷伯菌加速从甘油中生产1,3-丙二醇

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

1,3-Propanediol (1,3-PD) is a bivalent alcohol, used in a number of chemical syntheses. It could be produced from glycerol in course of microbial fermentation by Klebsiella pneumoniae along with more than five minor liquid products. With the purpose to enhance 1,3-PD production and to eliminate by-products formation, principally new pH control on the process was applied. The method, named "forced pH fluctuations" was realized by consecutive raisings of pH with definite ApH amplitude (ranging from 1.0 to 2.0) at time intervals between 2 and 4 h, during a series of fed batch processes. The fermentation performed by forced pH fluctuations with ApH = 1.0, risen at every 3 h was evaluated as the most successful. Increase by 10% of the maximal amount of 1,3-PD (g/1), 22% higher productivity [g/(l h)], and 29% increase in 1,3-PD molar yield were achieved, compared to the referent fed batch (with constant pH = 7.0). In addition, significant decrease in byproducts formation was obtained. The most important reduction was observed in the lactic and acetic acids yields, where 50 and 70% decrease were reached. The results suggested the potential of pH to manage the share and quantity of product spectrum in mixed diols-acids fermentations. The application of "forced pH fluctuations method" achieves the desirable increase in 1,3-PD formation and decrease in by-products accumulation at the same time by a comparatively simple approach by adjustment of one bioprocess parameter only.
机译：1,3-丙二醇（1,3-PD）是一种二价醇，用于许多化学合成中。它可以在肺炎克雷伯菌的微生物发酵过程中由甘油以及五种以上的次要液体产品制成。为了提高1,3-PD的产量并消除副产物的形成，主要在工艺中采用了新的pH控制方法。该方法称为“强制pH波动”，是通过在一系列补料分批过程中以2至4 h的时间间隔连续以一定的ApH幅度（1.0至2.0）升高pH来实现的。通过每3小时升高一次的ApH = 1.0的强制pH波动进行的发酵被认为是最成功的。与1,3-PD（g / 1）的最大量相比增加了10％，生产率[g /（lh）]提高了22％，与1,3-PD摩尔产率相比增加了29％。参照进料批次（恒定pH = 7.0）。另外，获得了副产物形成的显着减少。观察到最重要的降低是乳酸和乙酸的产率，分别降低了50％和70％。结果表明，在混合的二醇-酸发酵中，pH值有可能控制产物谱的份额和数量。 “强制pH波动法”的应用通过一种相对简单的方法，只需调整一个生物过程参数，即可同时实现1,3-PD形成的理想增加和副产物积累的减少。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号