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首页> 外文期刊>Bioprocess and Biosystems Engineering >Improved production of Pseudomonas sp. ECU1011 acetyl esterase by medium design and fed-batch fermentation
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Improved production of Pseudomonas sp. ECU1011 acetyl esterase by medium design and fed-batch fermentation

机译：假单胞菌属sp的产量提高。 ECU1011乙酰酯酶通过培养基设计和分批补料发酵

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摘要

We optimized culture medium and batch-fed fermentation conditions to enhance production of an acetyl esterase from Pseudomonas sp. ECU 1011 (PSAE). This enzyme enantioselectively deacetylates α-acetoxyphenyl-acetic acid. The medium was redesigned by single-factor and statistical optimization. The addition of ZnSO_4 enhanced enzyme production by 37%. Yeast extract concentration was directly associated with the enzyme production. The fermentation was scaled up in a 5-1 fermenter with the optimized medium, and the correlations between enzyme production and dissolved oxygen, pH, and feeding strategy were investigated. The fermentation process was highly oxygen-demanding, pH sensitive and mandelic acid-inducible. The fermentation pH was controlled at 7.5 by a pH and dissolved oxygen feedback strategy. Feeding mandelic acid as both a pH regulator and an enzyme inducer increased the enzyme production by 23%. The results of the medium redesign experiments were confirmed and explained in fed-batch culture experiments. Mathematical models describing the fermentation processes indicated that the enzyme production was strongly associated with cell growth. The optimized pH and dissolved oxygen stat fed-batch process resulted high volumetric production of PSAE (4166 U/l, 7.2-fold higher than the initial) without enantioselectivity decline. This process has potential applications for industrial production of chiral mandelic acid or its derivatives.
机译：我们优化了培养基和分批进料的发酵条件，以增强假单胞菌sp。乙酰酯酶的生产。 ECU 1011（PSAE）。该酶将α-乙酰氧基苯基乙酸对映体选择性地脱乙酰。通过单因素和统计优化对培养基进行了重新设计。 ZnSO_4的添加使酶的产量提高了37％。酵母提取物的浓度与酶的产生直接相关。在具有优化培养基的5-1发酵罐中扩大发酵规模，并研究酶的产生与溶解氧，pH和补料策略之间的相关性。发酵过程是高度需氧的，对pH敏感并且可诱导扁桃酸。通过pH和溶解氧反馈策略将发酵pH控制在7.5。饲喂扁桃酸作为pH调节剂和酶诱导剂，可使酶的产量增加23％。培养基重新设计实验的结果在分批补料培养实验中得到证实和解释。描述发酵过程的数学模型表明，酶的产生与细胞的生长密切相关。优化的pH和溶解氧稳态补料分批工艺可实现高产量的PSAE（4166 U / l，比初始产量高7.2倍），而对映选择性不降低。该方法对于工业生产手性扁桃酸或其衍生物具有潜在的应用。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2012年第3期|p.323-331|共9页
  • 作者

    Xin Ju; Hui-Lei Yu; Jiang Pan; Jian-He Xu;

  • 作者单位

    Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237 Shanghai, People's Republic of China;

    Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237 Shanghai, People's Republic of China;

    Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237 Shanghai, People's Republic of China;

    Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237 Shanghai, People's Republic of China;

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  • 正文语种 eng
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  • 关键词

    acetyl esterase; pseudomonas sp; medium redesign; fed-batch culture; pH and DO feedback control;

    机译：乙酰酯酶假单胞菌重新设计;分批补料培养pH和DO反馈控制;

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