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Mycogenesis of gold nanoparticles using a phytopathogen Alternaria alternata

机译:使用植物病原体链格孢菌金纳米颗粒的致真菌作用

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摘要

The development of an eco-friendly and reliable process for the synthesis of gold nanomaterials (AuNPs) using microorganisms is gaining importance in the field of nanotechnology. In the present study, AuNPs have been synthesized by bio-reduction of chloroauric acid (HAuCl_4) using the fungal culture filtrate (FCF) of Alternaria alternata. The synthesis of the AuNPs was monitored by UV-visible spectroscopy. The particles thereby obtained were characterized by UV, dynamic light scattering (DLS), X-ray diffraction (XRD), energy dispersive X-ray (EDX) analysis, Fourier transform infrared (FTIR) spectroscopy, atomic force microscopy (AFM) and transmission electron microscopy (TEM). Energy-dispersive X-ray study revealed the presence of gold in the nanoparticles. Fourier transform infrared spectroscopy confirmed the presence of a protein shell outside the nanoparticles which in turn also support their stabilization. Treatment of the fungal culture filtrate with aqueous Au~+ ions produced AuNPs with an average particle size of 12 ± 5 nm. This proposed mechanistic principal might serve as a set of design rule for the synthesis of nanostructures with desired architecture and can be amenable for the large scale commercial production and technical applications.
机译:利用微生物合成金纳米材料(AuNPs)的环保,可靠方法的开发在纳米技术领域中变得越来越重要。在本研究中,通过使用交替链霉菌的真菌培养滤液(FCF)对氯金酸(HAuCl_4)进行生物还原来合成AuNP。 AuNP的合成通过紫外-可见光谱法监测。通过紫外线,动态光散射(DLS),X射线衍射(XRD),能量色散X射线(EDX)分析,傅里叶变换红外(FTIR)光谱,原子力显微镜(AFM)和透射来表征由此获得的颗粒电子显微镜(TEM)。能量色散X射线研究表明纳米粒子中存在金。傅里叶变换红外光谱证实了在纳米颗粒外部存在蛋白质壳,这反过来也支持了它们的稳定性。用Au〜+离子水溶液处理真菌培养物滤液,产生平均粒径为12±5 nm的AuNP。提出的机制原理可以用作合成具有所需结构的纳米结构的一组设计规则,并且可以适用于大规模的商业生产和技术应用。

著录项

  • 来源
    《Bioprocess and Biosystems Engineering》 |2012年第4期|p.637-643|共7页
  • 作者单位

    Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany,University of Calcutta, Kolkata 700019, India;

    Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany,University of Calcutta, Kolkata 700019, India;

    Department of Polymer Science and Technology,University College of Science and Technology,University of Calcutta, Kolkata 700009, India;

    Transmission Electron Microscopy Section (Infectious Disease and Immunology), Indian Institute of Chemical Biology,Kolkata 700032, India;

    Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany,University of Calcutta, Kolkata 700019, India;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    alternaria alternata; fungal culture filtrate; gold nano;

    机译:链格孢;真菌培养滤液;金纳米;

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