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首页> 外文期刊>Bioprocess and Biosystems Engineering >Sugar uptake analysis of suspension Arabidopsis, tobacco, and rice cells in various media using an FT-IR/ATR method
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Sugar uptake analysis of suspension Arabidopsis, tobacco, and rice cells in various media using an FT-IR/ATR method

机译：使用FT-IR / ATR方法在各种培养基中分析悬浮拟南芥，烟草和水稻细胞的糖吸收

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The kinetic behavior of the sugar uptake phenomena of a suspension of Arabidopsis cells was investigated by mid-infrared spectroscopy using Fourier transform infrared spectrometers and attenuated total reflection techniques. The kinetic behavior of the cell growth was also studied and the growth and the sugar uptake behaviors were discussed for three typical plant cells (Arabidopsis, TBY-2, and rice cells). The cell growth rate and the lag period were influenced by not only the types of the plant cells, but also the sugar species used as the carbon source. The characteristics of the sugar uptake behavior were clarified based on the difference in the three types of plant cells. The cell growth and the sugar uptake progressed at approximately the same time in the TBY-2 cells. In the rice cells, the sugar uptake rate was relatively lower than that of the others. On the other hand, the sugar uptake of the Arabidopsis cells started before the cell growth. Furthermore, glucose as the carbon source of the Arabidopsis cell cultivation seems to significantly influence the sugar metabolism. Glucose had a significant influence on the sugar metabolism of the other sugar under the conditions for the mixture of glucose and the other sugar. The characteristics of the sugar uptake phenomena based on the cell growth stage was typical for each plant cell except for some sugars, such as galactose and tre-halose, and the behavior of the total sugar uptake had not changed. These results suggested that the cell growth and the sugar uptake in the plant cell cultivation processes may be controlled by the combined supply of the sugar species as the carbon source. The detailed data for plant cell cultivation using each sugar obtained in this study would be useful for bioscience research and for cultivation process control using various sugars, for example, purified or sugar mixtures formed from biomass materials.
机译：通过使用傅立叶变换红外光谱仪和衰减全反射技术的中红外光谱研究了拟南芥细胞悬浮液的糖吸收现象的动力学行为。还研究了细胞生长的动力学行为，并讨论了三种典型植物细胞（拟南芥，TBY-2和水稻细胞）的生长和糖吸收行为。细胞的生长速度和滞后期不仅受植物细胞类型的影响，还受用作碳源的糖种的影响。根据三种类型植物细胞的差异，阐明了糖的摄取行为特征。在TBY-2细胞中，细胞生长和糖摄取大约同时进行。在水稻细胞中，糖的吸收率相对低于其他细胞。另一方面，拟南芥细胞的糖摄取在细胞生长之前就开始了。此外，葡萄糖作为拟南芥细胞培养的碳源似乎显着影响糖的代谢。在葡萄糖和另一种糖的混合物的条件下，葡萄糖对另一种糖的糖代谢具有显着影响。除某些糖（例如半乳糖和海藻糖）外，每种植物细胞都具有基于细胞生长阶段的糖吸收现象的特征，并且总糖吸收的行为没有改变。这些结果表明，植物细胞培养过程中细胞的生长和糖的吸收可以通过糖源作为碳源的联合供应来控制。使用本研究中获得的每种糖进行植物细胞培养的详细数据，对于生物科学研究和使用各种糖（例如，由生物质材料形成的纯化糖或糖混合物）的栽培过程控制都是有用的。

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