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首页> 外文期刊>Bioprocess and Biosystems Engineering >Utilization of Jatropha deoiled seed cake for production of cellulases under solid-state fermentation
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Utilization of Jatropha deoiled seed cake for production of cellulases under solid-state fermentation

机译：利用麻疯树脱油的种子饼在固态发酵下生产纤维素酶

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摘要

Toxic waste generated by Jatropha seed cake after utilization of biodiesel on one hand has stimulated the need to develop new technologies to treat the waste and on the other, forced us to reevaluate the efficient utilization of its nutritive potential for production of various high-value compounds and its conversion to non-toxic forms which could be used as animal feed stock. In this study, Jatropha seed cake was used for production of cellulases by new isolate of Thermoascus aurantiacus under solid-state fermentation. The interaction of nitrogen source concentration, moisture ratio, initial pH of the medium and inoculum size was investigated and modelled using response surface methodology (RSM) using Box-Behnken Design (BBD). Under optimized conditions endo-β-1,4-glucanase, β-glu-cosidase and filter paper activities were found to be 124.44, 28.86, 4.87 U/g of substrate, respectively. Characterization of endo-β-l,4-glucanase, β-glucosidase was done after partial purification by ammonium sulfate fractionation followed by desalting. The endo-β-1,4-glucanase and β-glucosidase showed maximum activity at 70 ℃ and pH 4. Saccharification studies performed with different ligno-cellulosic substrates showed that sugar cane bagasse was most susceptible to enzymatic hydrolysis. The study suggests that Jatropha seed cake can be used as a viable nutrient source for cellulase production without any pre-treatment under solid-state fermentation by T. aurantiacus.
机译：麻疯树籽饼在利用生物柴油后产生的有毒废物一方面刺激了开发处理废物的新技术的需求，另一方面迫使我们重新评估其营养潜力在生产各种高价值化合物方面的有效利用并将其转化为无毒形式，可用作动物饲料。在这项研究中，麻风树种子饼用于固态发酵下新的嗜热曲霉分离株的生产纤维素酶。使用Box-Behnken Design（BBD），使用响应面方法（RSM）对氮源浓度，水分比，培养基的初始pH和接种量的相互作用进行了调查和建模。在优化的条件下，发现内切β-1,4-葡聚糖酶，内切葡聚糖酶和滤纸活性分别为底物的124.44、28.86、4.87 U / g。在通过硫酸铵分级分离，随后脱盐进行部分纯化之后，对内-β-1,4-葡聚糖酶，β-葡糖苷酶进行表征。内切β-1,4-葡聚糖酶和β-葡糖苷酶在70℃和pH 4时显示出最大的活性。对不同木质纤维素底物的糖化研究表明，甘蔗渣最容易被酶水解。该研究表明，麻风树种子饼可以用作生产纤维素酶的可行营养源，而无需在金黄色葡萄球菌固态发酵条件下进行任何预处理。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2012年第8期|p.1343-1353|共11页
  • 作者

    Bhaumik R. Dave; Ankit P. Sudhir; Mehul Pansuriya; Dharmesh P. Raykundaliya; R. B. Subramanian;

  • 作者单位

    BRD School of Biosciences, Sardar Patel University,Sardar Patel Maidan, Vadtal Road, Satellite Campus,PO Box 39, Vallabh Vidyanagar 388 120, Gujarat, India;

    BRD School of Biosciences, Sardar Patel University,Sardar Patel Maidan, Vadtal Road, Satellite Campus,PO Box 39, Vallabh Vidyanagar 388 120, Gujarat, India;

    BRD School of Biosciences, Sardar Patel University,Sardar Patel Maidan, Vadtal Road, Satellite Campus,PO Box 39, Vallabh Vidyanagar 388 120, Gujarat, India;

    Department of Statistics, Sardar Patel University,Vallabh Vidyanagar 388 120, Gujarat, India;

    BRD School of Biosciences, Sardar Patel University,Sardar Patel Maidan, Vadtal Road, Satellite Campus,PO Box 39, Vallabh Vidyanagar 388 120, Gujarat, India;

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  • 关键词

    jatropha seed cake; cellulases; thermoascus aurantiacus; optimization saccharification; solid state;

    机译：麻风树籽饼;纤维素酶;黄曲霉;优化糖化;固态;

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