...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Bioprocess and Biosystems Engineering >Isolation of enantioselective α-hydroxyacid dehydrogenases based on a high-throughput screening method
【24h】

Isolation of enantioselective α-hydroxyacid dehydrogenases based on a high-throughput screening method

机译：基于高通量筛选方法的对映选择性α-羟酸脱氢酶的分离

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To isolate enantioselective a-hydroxyacid dehydrogenases (α-HADHs), a high-throughput screening method was established. 2,4-Dinitrophenylhydrazine solution forms a red-brown complex with ketoacid produced during the a-HADH-mediated oxidation of α-hydroxyacid. The complex can be easily quantified by spectrophoto-metric measurement at 458 nm. The enantioselectivity of α-HADH in each strain can be measured with this colorimetric method using (R)- and (S)-α-hydroxyacid concurrently as substrates to evaluate the apparent enantioselectivity (E_(app))-The E_(app) closely matches the value of true enantioselectivity (E_(app)) determined by HPLC analysis. With this method, a total of 34 stains harboring enantioselective a-HADHs were selected from 526 potential a-HADH-producing microorganisms. Pseudomonas aeruginosa displayed the highest (5)-enantioselective a-HADH activity. This strain appears promising for potential application in industry to produce (R)-α-hydroxyacids. The method described herein represents a useful tool for the high-throughput isolation of enantioselective α-HADHs.
机译：为了分离对映选择性α-羟酸脱氢酶（α-HADHs），建立了高通量筛选方法。 2,4-二硝基苯肼溶液与α-羟乙酸在α-HADH介导的氧化过程中生成的酮酸形成红棕色络合物。该络合物可以通过分光光度法在458 nm处进行定量。可以使用该比色法同时使用（R）-和（S）-α-羟酸作为底物来评估每个菌株中α-HADH的对映选择性，以密切评估表观对映选择性（E_（app））-E_（app）与通过HPLC分析确定的真实对映选择性（E_（app））值匹配。用这种方法，从526种潜在的产生α-HADH的微生物中选择了总共34种带有对映选择性α-HADH的染色剂。铜绿假单胞菌表现出最高的（5）-对映选择性a-HADH活性。该菌株似乎有望在工业上用于生产（R）-α-羟基酸。本文所述的方法代表了用于高通量分离对映选择性α-HADH的有用工具。

著录项

  • 来源
    《Bioprocess and Biosystems Engineering》 |2012年第9期|p.1515-1522|共8页
  • 作者

    Ya-Ping Xue; Wei Wang; Ya-Jun Wang; Zhi-Qiang Liu; Yu-Guo Zheng; Yin-Chu Shen;

  • 作者单位

    Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, People's Republic of China,Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou 310014, People's Republic of China;

    Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, People's Republic of China,Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou 310014, People's Republic of China;

    Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, People's Republic of China,Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou 310014, People's Republic of China;

    Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, People's Republic of China,Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou 310014, People's Republic of China;

    Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, People's Republic of China,Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou 310014, People's Republic of China;

    Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, People's Republic of China,Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou 310014, People's Republic of China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    high-throughput screening; α-hydroxyacid; α-hydroxyacid dehydrogenase; enantioselectivity; pseudomonas aeruginosa;

    机译：高通量筛选α-羟酸;α-羟酸脱氢酶;对映选择性铜绿假单胞菌;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号