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首页> 外文期刊>Bioprocess and Biosystems Engineering >Optimization of saccharification and ethanol production by simultaneous saccharification and fermentation (SSF) from seaweed, Saccharina japonica
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Optimization of saccharification and ethanol production by simultaneous saccharification and fermentation (SSF) from seaweed, Saccharina japonica

机译:通过同时进行糖化和发酵(SSF)的海藻,日本糖衣藻的糖化和乙醇生产的优化

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Ethanol was produced using the simultaneous saccharification and fermentation (SSF) method with mac-roalgae polysaccharide from the seaweed Saccharina japonica (Sea tangle, Dasima) as biomass. The seaweed was dried by hot air, ground with a hammer mill and filtered with a 200-mesh sieve prior to pretreatment. Saccharification was carried out by thermal acid hydrolysis with H_2SO_4 and the industrial enzyme, Termamyl 120 L. To increase the yield of saccharification, isolated marine bacteria were used; the optimal saccharification conditions were 10% (w/v) seaweed slurry, 40 mM H_2SO_4 and 1 g dcw/L isolated Bacillus sp. JS-1. Using this saccharification procedure, the reducing sugar concentration and viscosity were 45.6 ± 5.0 g/L and 24.9 cp, respectively, and the total yield of the saccharification with optimal conditions and S. japonica was 69.1%. Simultaneous saccharification and fermentation was carried out for ethanol production. The highest ethanol concentration, 7.7 g/L (9.8 ml/L) with a theoretical yield of 33.3%, was obtained by SSF with 0.39 g dcw/L Bacillus sp. JS-1 and 0.45 g dcw/L of the yeast, Pichia angophorae KCTC 17574.
机译:使用同步糖化和发酵(SSF)方法,以海藻Saccharina japonica(Sea tangle,Dasima)的mac-roalgae多糖为生物质生产乙醇。将海藻用热空气干燥,用锤磨机研磨,然后在预处理之前用200目筛子过滤。通过用H_2SO_4和工业酶Termamyl 120 L进行热酸水解来进行糖化。为提高糖化的产量,使用了分离的海洋细菌。最佳糖化条件为10%(w / v)海藻浆,40 mM H_2SO_4和1 g dcw / L分离的芽孢杆菌。 JS-1。使用该糖化程序,还原糖浓度和粘度分别为45.6±5.0 g / L和24.9 cp,并且在最佳条件和粳稻的条件下糖化的总产率为69.1%。同时进行糖化和发酵以生产乙醇。 SSF用0.39 g dcw / L芽孢杆菌属菌株获得的最高乙醇浓度为7.7 g / L(9.8 ml / L),理论产率为33.3%。 JS-1和0.45 g dcw / L的酵母,毕赤酵母KCTC 17574。

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