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首页> 外文期刊>Bioprocess and Biosystems Engineering >Immobilization of cellulase from newly isolated strain Bacillus subtilis TD6 using calcium alginate as a support material
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Immobilization of cellulase from newly isolated strain Bacillus subtilis TD6 using calcium alginate as a support material

机译：使用藻酸钙作为支撑材料固定新分离的枯草芽孢杆菌TD6菌株的纤维素酶

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摘要

Bacillus subtilis TD6 was isolated from Taki-fugu rubripes, also known as puffer fish. Cellulase from this strain was partially purified by ammonium sulphate precipitation up to 80% saturation, entrapped in calcium alginate beads, and finally characterized using CMC as the substrate. For optimization, various parameters were observed, including pH maximum, temperature maximum, sodium alginate, and calcium chloride concentration. pH maximum of the enzyme showed no changes before and after immobilization and remained stable at 6.0. The temperature maximum showed a slight increase to 60 ℃. Two percent sodium alginate and a 0.15 M calcium chloride solution were the optimum conditions for acquisition of enzyme with greater stability. K_m and V_(max) values for the immobilized enzyme were slightly increased, compared with those of free enzyme, 2.9 mg/ml and 32.1 μmol/min/ mL, respectively. As the purpose of immobilization, reusability and storage stability of the enzyme were also observed. Immobilized enzyme retained its activity for a longer period of time and can be reused up to four times. The storage stability of entrapped cellulase at 4 ℃ was found to be up to 12 days, while at 30 ℃, the enzyme lost its activity within 3 days.
机译：枯草芽孢杆菌TD6是从也称为河豚鱼的Taki-fugu rubripes中分离得到的。来自该菌株的纤维素酶通过硫酸铵沉淀至80％的饱和度进行部分纯化，包埋在藻酸钙珠粒中，最后使用CMC作为底物进行表征。为了优化，观察到各种参数，包括最大pH，最大温度，藻酸钠和氯化钙浓度。该酶的最大pH值在固定前后没有变化，并保持稳定在6.0。最高温度略有上升至60℃。 2％的海藻酸钠和0.15 M的氯化钙溶液是获得稳定性更高的酶的最佳条件。与游离酶相比，固定化酶的K_m和V_（max）值分别略有增加，分别为2.9 mg / ml和32.1μmol/ min / mL。作为固定的目的，还观察到了酶的可重复使用性和储存稳定性。固定化酶可以长时间保持其活性，最多可以重复使用四次。截留的纤维素酶在4℃下的储存稳定性长达12天，而在30℃时，该酶在3天内失去了活性。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2012年第2期|p.29-33|共5页
  • 作者

    Dian Andriani; Changshin Sunwoo; Hwa-Won Ryu; Bambang Prasetya; Don-Hee Park;

  • 作者单位

    Interdisciplinary Program of Graduate School for Bioenergy and Biomaterials, Chonnam National University,Gwangju 500-757, South Korea,Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Bogor 16911, Indonesia;

    Interdisciplinary Program of Graduate School for Bioenergy and Biomaterials, Chonnam National University,Gwangju 500-757, South Korea,School of Biological Sciences and Technology,Chonnam National University, Gwangju 500-757, South Korea;

    Interdisciplinary Program of Graduate School for Bioenergy and Biomaterials, Chonnam National University,Gwangju 500-757, South Korea,School of Biological Sciences and Technology,Chonnam National University, Gwangju 500-757, South Korea;

    Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Bogor 16911, Indonesia;

    Interdisciplinary Program of Graduate School for Bioenergy and Biomaterials, Chonnam National University,Gwangju 500-757, South Korea,School of Biological Sciences and Technology,Chonnam National University, Gwangju 500-757, South Korea,Research Institute for Catalyst, Chonnam National University,Gwangju 500-757, South Korea;

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  • 正文语种 eng
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  • 关键词

    immobilization; cellulase; calcium alginate; bacillus subtilis TD6; takifugu rubripes;

    机译：固定纤维素酶海藻酸钙;枯草芽孢杆菌TD6;aki鱼;

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