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A novel separation and enrichment method of 17β-estradiol using aptamer-anchored microbeads

机译：适体锚定微珠分离富集17β-雌二醇的新方法

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摘要

The estrogenic compound 17β-estradiol (E2) is widely studied for its potential endocrine disruption effects. Due to the low level of E2 present in the environment, it is highly desirable to develop a sensitive and efficient separation and enrichment method for E2 analysis. In this paper, we proposed a novel E2 preconcentration method using anti-E2 aptamer-anchored isothiocyanate-modified beads (NCS beads). The glass beads are chemically modified with primary amino group, and then treated with phenylene diisothiocyanate (PDITC) to generate an iso-thiocyanate group, which is reactive towards the amine group. The amino-modified anti-E2 aptamer can be easily covalently immobilized onto the as-prepared NCS beads. The experimental results demonstrated that the aptamer affinity microbeads could selectively retain and separate E2 compound. The effects of the operation parameters on retention of E2, including washing condition, eluting condition, the number of beads, and the incubation time were investigated. Moreover, high-performance liquid chroma-tography with preconcentration of E2 on the aptamer affinity microbeads was applied to detect the E2 in the spiked water samples and obtained a good recovery.
机译：广泛研究雌激素化合物17β-雌二醇（E2）的潜在内分泌干扰作用。由于环境中存在的E2含量较低，因此非常需要开发一种灵敏而有效的E2分析分离和富集方法。在本文中，我们提出了一种使用抗E2适体锚定的异硫氰酸酯修饰的磁珠（NCS磁珠）的新型E2富集方法。用伯氨基对玻璃珠进行化学修饰，然后用亚苯基二异硫氰酸酯（PDITC）处理以生成异硫氰酸酯基，该异硫氰酸酯基对胺基具有反应性。氨基修饰的抗E2适体可以很容易地共价固定在制备好的NCS珠上。实验结果表明，适体亲和力微珠可以选择性地保留和分离E2化合物。研究了操作参数对E2保留的影响，包括洗涤条件，洗脱条件，微珠数量和孵育时间。此外，采用在适体亲和微珠上预浓缩了E2的高效液相色谱法检测加标水样中的E2，并获得了良好的回收率。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2011年第2期|p.189-195|共7页
  • 作者

    Gioi Dong Huy; Na Jin; Bin-Cheng Yin; Bang-Ce Ye;

  • 作者单位

    Lab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Meilong Road 130, Shanghai 200237, China;

    Lab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Meilong Road 130, Shanghai 200237, China;

    Lab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Meilong Road 130, Shanghai 200237, China;

    Lab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Meilong Road 130, Shanghai 200237, China;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    17β-estradiol; separation; enrichment; affinity chromatography; aptamer;

    机译：17β-雌二醇;分离丰富;亲和色谱适体;

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