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首页> 外文期刊>Bioprocess and Biosystems Engineering >Microfiltration conditions modify Lactobacillus bulgaricus cryotolerance in response to physiological changes
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Microfiltration conditions modify Lactobacillus bulgaricus cryotolerance in response to physiological changes

机译：微滤条件响应生理变化而改变了保加利亚乳杆菌的耐低温性

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摘要

This work aimed at analyzing the effect of microfiltration conditions (cross-flow velocity and trans-membrane pressure) on the quality of frozen Lactobacillus bulgaricus CFL1 starters produced on pilot scale. Micro-filtered cells were less resistant during the concentration process than centrifuged cells. In contrast, bacterial cryotolerance during freezing was improved after microfiltration, in a range of 28-88%, depending on the microfiltration conditions. During frozen storage, cell resistance was also affected by microfiltration conditions, either positively or negatively, compared to centrifugation. The best cryotolerance was obtained for cells microfiltered at a cross-flow velocity of 2 m/s and a transmembrane pressure of 0.15 MPa. This improvement was explained by considering membrane fatty acid composition of Lb. bulgaricus CFL1. This condition increased unsaturated to saturated and cyclic to saturated fatty acid ratios, which enhanced membrane fluidity, thus helping the cells to better resist freezing and frozen storage.
机译：这项工作旨在分析微滤条件（横流速度和跨膜压力）对中试规模生产的保加利亚保加利亚乳杆菌CFL1发酵剂的品质的影响。在浓缩过程中，微滤细胞的抗性不如离心细胞。相反，根据微滤条件，微滤后冷冻过程中的细菌低温耐受性得到改善，范围为28-88％。在冷冻保存过程中，与离心相比，微滤条件对细胞的抵抗力也有正面或负面的影响。对于以2 m / s的错流速度和0.15 MPa的跨膜压力进行微滤的细胞，可获得最佳的耐低温性。通过考虑Lb的膜脂肪酸组成解释了这种改善。保加利亚CFL1。这种条件增加了不饱和脂肪酸与饱和脂肪酸以及环状脂肪酸与饱和脂肪酸的比率，从而提高了膜的流动性，从而帮助细胞更好地抵抗冷冻和冷冻保存。

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