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首页> 外文期刊>Bioprocess and Biosystems Engineering >Direct electron transfer and electrochemical study of hemoglobin immobilized in ZnO hollow spheres
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Direct electron transfer and electrochemical study of hemoglobin immobilized in ZnO hollow spheres

机译：固定在ZnO空心球中的血红蛋白的直接电子转移和电化学研究

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摘要

ZnO hollow spheres were firstly prepared. A new type of amperometric hydrogen peroxide biosensor was fabricated by entrapping Hemoglobin (Hb) through the ZnO hollow spheres (ZHS) nanoparticles. The composition morphology and size were studied by transmission electron microscopy. The surface topography of the prepared films was imaged by atomic force microscope (AFM). Several techniques, including UV-vis absorption spectroscopy, cyclic voltammetry, chronoamperometry were employed to characterize the performance of the biosensor. The results indicated that the ZHS nanoparticles had enhanced the performance of the hydrogen peroxide sensors. The electrochemical parameters of Hb in the ZHS were calculated by the results of the electron-transfer coefficient (α) and the apparent heterogeneous electron-transfer rate constant K_s as 0.5 and 3.1 s~(-1) respectively. The resulting biosensors showed a wide linear range from 2.1 × 10~(-6) to 5.18 × 10~(-3) M, with a low detection limit of 7.0 × 10~(-7) M (S/N = 3) under optimized experimental conditions. The results demonstrated that the ZHS matrix may improve the protein loading with the retention of bioactivity and greatly promote the direct electron transfer, which can be attributed to its unique morphology, high specific surface area, and biocompatibility. The biosensor obtained from this study possesses high sensitivity, good reproducibility, and long-term stability.
机译：首先制备了ZnO空心球。通过将血红蛋白（Hb）截留在ZnO空心球（ZHS）纳米颗粒中，制得了一种新型的安培型过氧化氢生物传感器。通过透射电子显微镜研究了组成形态和尺寸。制备的膜的表面形貌通过原子力显微镜（AFM）成像。包括紫外可见吸收光谱法，循环伏安法，计时电流法在内的几种技术被用来表征生物传感器的性能。结果表明，ZHS纳米颗粒增强了过氧化氢传感器的性能。通过电子传递系数（α）和表观异质电子传递速率常数K_s分别为0.5和3.1 s〜（-1）计算出ZHS中Hb的电化学参数。所得的生物传感器显示出从2.1×10〜（-6）到5.18×10〜（-3）M的宽线性范围，低检测限为7.0×10〜（-7）M（S / N = 3）在优化的实验条件下结果表明，ZHS基质可以改善蛋白质载量并保持生物活性，并大大促进直接电子转移，这可以归因于其独特的形态，高比表面积和生物相容性。从这项研究中获得的生物传感器具有高灵敏度，良好的可重复性和长期稳定性。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2011年第8期|p.931-938|共8页
  • 作者

    Changhua Liu; Jing Xu; Zongfang Wu;

  • 作者单位

    Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400700, People's Republic of China;

    Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400700, People's Republic of China;

    Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400700, People's Republic of China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    biosensor; direct electron transfer; ZnO hollow spheres; hemoglobin;

    机译：生物传感器直接电子转移ZnO空心球;血红蛋白;

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