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首页> 外文期刊>Bioprocess and Biosystems Engineering >Optimization of fibrolytic enzyme production by Aspergillus japonicus C03 with potential application in ruminant feed and their effects on tropical forages hydrolysis
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Optimization of fibrolytic enzyme production by Aspergillus japonicus C03 with potential application in ruminant feed and their effects on tropical forages hydrolysis

机译：日本曲霉C03产纤溶酶的优化及其在反刍动物饲料中的潜在应用及其对热带草料水解的影响

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摘要

Fibrolytic enzyme production by Aspergillus japonicus C03 was optimized in a medium containing agro-industrial wastes, supplemented with peptone and yeast extract. A 2~3 full factorial composite and response surface methodology were used to design the experiments and analysis of results. Tropical forages were hydrolyzed by A. japonicus C03 enzymatic extract in different levels, and they were also tested as enzymatic substrate. Optimal production to xylanase was obtained with soybean bran added to crushed corncob (1:3), 0.01% peptone, and 0.2% yeast extract, initial pH 5.0, at 30 ℃ under static conditions for 5 days of incubation. Optimal endoglucanase production was obtained with wheat bran added to sugarcane bagasse (3:1), 0.01% peptone, and 0.2% yeast extract, initial pH 4.0, at 30 ℃, for 6 days, under static conditions. Addition of nitrogen sources as ammonium salts either inhibited or did not influence xylanase production. This enzymatic extract had a good result on tropical forage hydrolyzes and showed better performance in the Brachiaria genera, due to their low cell wall lignin quantity. These results represent a step forward toward the use of low-cost agricultural residues for the production of valuable enzymes with potential application in animal feed, using fermentation conditions.
机译：在含有农用工业废料，并补充蛋白ept和酵母提取物的培养基中，优化了日本曲霉C03产生的纤溶酶。采用2〜3个全因子复合材料和响应面法进行实验设计和结果分析。热带草料被不同程度的日本刺参CO 3酶促提取物水解，并作为酶促底物进行了测试。在静态条件下于30℃下孵育5天，将大豆糠加入压碎的玉米芯（1：3），0.01％的蛋白ept和0.2％的酵母提取物中，初始pH为5.0，可获得最佳的木聚糖酶产量。在静态条件下，在30℃下，将初始麦芽汁添加到甘蔗渣（3：1），0.01％蛋白，和0.2％酵母提取物中（初始pH 4.0），可实现最佳的内切葡聚糖酶生产。氮源作为铵盐的添加抑制或不影响木聚糖酶的产生。该酶提取物对热带草料水解具有良好的效果，并且由于其细胞壁木质素含量低，因此在腕带属中表现出更好的性能。这些结果代表了朝着使用低成本农业残留物生产有价值的酶迈出的一步，并利用发酵条件将其潜在地应用于动物饲料。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2011年第8期|p.1027-1038|共12页
  • 作者

    Fernanda D. A. Facchini; Ana C. Vici; Vivian M. Benassi; Luiz A. P. Freitas; Ricardo A. Reis; Joao A. Jorge; Hector F. Terenzi; Maria de Lourdes T. M. Polizeli;

  • 作者单位

    Departamento de Bioqufmica e Imunologia, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Sao Paulo, Brazil;

    Departamento de Bioqufmica e Imunologia, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Sao Paulo, Brazil;

    Departamento de Bioqufmica e Imunologia, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Sao Paulo, Brazil;

    Departamento de Ciencias Farmaceuticas, Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Sao Paulo, Brazil;

    Departamento de Zootecnia, Faculdade Ciencias Agrarias e Veterinarias, Universidade Estadual Paulista 'Julio Mesquita Filho', Sao Paulo, Brazil;

    Departamento de Biologia, Faculdade de Filosofia Ciencias e Letras de Ribeirao Preto, Universidade de Sao Paulo, Av. Bandeirantes, 3900, Ribeirao Preto, SP 13040-901, Brazil;

    Departamento de Biologia, Faculdade de Filosofia Ciencias e Letras de Ribeirao Preto, Universidade de Sao Paulo, Av. Bandeirantes, 3900, Ribeirao Preto, SP 13040-901, Brazil;

    Departamento de Biologia, Faculdade de Filosofia Ciencias e Letras de Ribeirao Preto, Universidade de Sao Paulo, Av. Bandeirantes, 3900, Ribeirao Preto, SP 13040-901, Brazil;

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  • 正文语种 eng
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  • 关键词

    fibrolytic enzymes; aspergillus japonicus C03; response surface methodology; tropical forages; pretreatment;

    机译：纤溶酶;日本曲霉C03;响应面方法热带牧草;预处理;

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