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β-Glucosidase coating on polymer nanofibers for improved cellulosic ethanol production

机译:聚合物纳米纤维上的β-葡萄糖苷酶涂层可改善纤维素乙醇的生产

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摘要

β-Glucosidase (βG) can relieve the product inhibition of cellobiose in the cellulosic ethanol production by converting cellobiose into glucose. For the potential recycled uses, βG was immobilized and stabilized in the form of enzyme coating on polymer nanofibers. The βG coating (EC-βG) was fabricated by crosslinking additional βG molecules onto covalently attached βG molecules (CA-βG) via glutaraldehyde treatment. The initial activity of EC-βG was 36 times higher than that of CA-βG. After 20 days of incubation under shaking, CA-βG and EC-βG retained 33 and 91 % of each initial activity, respectively. Magnetic nanofibers were also used for easy recovery and recycled uses of βG coating. It is anticipated that the recycled uses of highly active and stable βG coating can improve the economics of cellulosic ethanol production sornlong as economical materials are employed as a host of enzyme immobilization.
机译:通过将纤维二糖转化为葡萄糖,β-葡萄糖苷酶(βG)可以减轻纤维素乙醇生产中纤维二糖的产物抑制。对于潜在的回收利用,βG以酶涂层的形式固定并稳定在聚合物纳米纤维上。 βG涂层(EC-βG)是通过戊二醛处理将其他βG分子交联到共价连接的βG分子(CA-βG)上而制成的。 EC-βG的初始活性是CA-βG的初始活性的36倍。摇动孵育20天后,CA-βG和EC-βG分别保留了每种初始活性的33%和91%。磁性纳米纤维还用于轻松回收和循环利用βG涂层。可以预期,由于经济的材料被用作酶固定的主体,因此高活性和稳定的βG涂层的循环利用可以改善纤维素乙醇生产的经济性。

著录项

  • 来源
    《Bioprocess and Biosystems Engineering》 |2010年第1期|141-147|共7页
  • 作者单位

    Department of Biological Engineering, Inha University, Inchon 402-751, Korea;

    Department of Chemical Engineering, Chosun University, Kwangju 501-759, Korea;

    Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Korea;

    College of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea;

    National Creative Research Initiative Center for Oxide Nanocrystalline Materials, School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Korea;

    National Creative Research Initiative Center for Oxide Nanocrystalline Materials, School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Korea;

    Department of Biological Engineering, Inha University, Inchon 402-751, Korea;

    Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Korea;

    Department of Chemical Engineering, Chosun University, Kwangju 501-759, Korea;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    cellulosic ethanol; β-Glucosidase; enzyme coating; electrospun nanofibers; magnetic nanoparticles;

    机译:纤维素乙醇β-葡萄糖苷酶;酶涂层电纺纳米纤维;磁性纳米粒子;

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