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首页> 外文期刊>Bioprocess and Biosystems Engineering >Improved cellulosic ethanol production from corn stover with a low cellulase input using a β-glucosidase-producing yeast following a dry biorefining process
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Improved cellulosic ethanol production from corn stover with a low cellulase input using a β-glucosidase-producing yeast following a dry biorefining process

机译：经过干法生物精制工艺后，使用产生β-葡萄糖苷酶的酵母，以低纤维素酶输入提高了玉米秸秆的纤维素乙醇生产

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摘要

A low-cost and sustainable cellulosic ethanol production is vital for fermentation-based industrial applications. Reducing the expenses of cellulose-deconstruction enzymes is one of the significant challenges to economic cellulose-to-ethanol conversion. Here, we report the improved ethanol production from corn stover after dry biorefining using a natural beta-glucosidase-producing strain Clavispora NRRL Y-50464 with a low cellulase dose of 5 mg protein/g glucan under separate enzymatic hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) conditions. Strain Clavispora NRRL Y-50464 exhibited a superior ethanol fermentation performance over Saccharomyces cerevisiae DQ1 under both conditions. It produced an ethanol titer of 38.1g/L within 96h at a conversion efficiency of 55.5% with 25% solids loading (w/w) via SSF without addition of extra beta-glucosidase supplement. Improved performance of Y-50464 on a bioreactor with a helical stirring apparatus confirmed its advantage over the conventional bioreactors originally designed for liquid fermentations in cellulosic ethanol conversion by SSF. The results of this study suggested that the strain Clavispora NRRL Y-50464 has a potential as a candidate for lower-cost cellulosic ethanol production from lignocellulosic materials.
机译：低成本和可持续的纤维素乙醇生产对于基于发酵的工业应用至关重要。减少纤维素解构酶的费用是经济地将纤维素转化为乙醇的重大挑战之一。在这里，我们报告了在使用单独的酶促水解和发酵（SHF）的情况下，使用天然β-葡萄糖苷酶生产菌株Clavispora NRRL Y-50464，纤维素酶剂量低至5 mg蛋白/ g葡聚糖，进行干法生物精炼后，玉米秸秆中乙醇的产量得到了改善。同时糖化和发酵（SSF）条件。在两种条件下，菌株克拉维孢菌NRRL Y-50464均显示出优于啤酒酵母DQ1的乙醇发酵性能。它通过SSF在96小时内产生的乙醇滴度为38.1g / L，转化效率为55.5％，固体含量为25％（w / w），而无需添加额外的β-葡萄糖苷酶补充剂。带有螺旋搅拌装置的Y-50464在生物反应器上的性能改善，证实了其优于最初设计用于SSF纤维素乙醇转化中液体发酵的常规生物反应器的优势。这项研究的结果表明，菌株Clavispora NRRL Y-50464有潜力作为从木质纤维素材料生产低成本纤维素乙醇的候选者。

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