Glycerol triacetate as solvent and acyl donor in the production of isoamyl acetate with Candida antarctica lipase B

Adi Wolfson; Aviad Atyya; Christina Dlugy; Dorith Tavor

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Glycerol triacetate as solvent and acyl donor in the production of isoamyl acetate with Candida antarctica lipase B

机译：三乙酸甘油酯作为溶剂和酰基供体在南极假丝酵母脂肪酶B生产乙酸异戊酯中

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Glycerol triacetate was successfully used as a green solvent and as the acyl donor in the transesterifica-tion of isoamyl alcohol to produce isoamyl acetate using free and immobilized Candida antarctica lipase B. Immobilized lipase was more catalytically active than free lipase and could be easily separated from the reaction mixture by filtration. In addition, it was found that increasing either the reaction temperature or the enzyme to substrate ratio increased the conversion of isoamyl alcohol. Using triacetin as the solvent also enabled the separation of product by simple extraction with petroleum ether and catalyst recycling.

机译：三乙酸甘油酯已成功地用作绿色溶剂和用作异戊醇酯交换反应的酰基供体，使用游离的和固定化的南极假丝酵母脂肪酶B生产乙酸异戊酯。固定化的脂肪酶比游离的脂肪酶更具催化活性，可以很容易地与通过过滤反应混合物。另外，发现增加反应温度或增加酶与底物的比率都增加了异戊醇的转化率。使用三醋精作为溶剂还可以通过用石油醚简单萃取和催化剂循环利用来分离产物。

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来源
《Bioprocess and Biosystems Engineering》 |2010年第3期|363-366|共4页
作者
Adi Wolfson; Aviad Atyya; Christina Dlugy; Dorith Tavor;
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作者单位

Green Processes Center, Chemical Engineering Department, Sami Shamoon College of Engineering, Bialik/Basel Sts., 84100 Beer Sheva, Israel;

Green Processes Center, Chemical Engineering Department, Sami Shamoon College of Engineering, Bialik/Basel Sts., 84100 Beer Sheva, Israel;

Green Processes Center, Chemical Engineering Department, Sami Shamoon College of Engineering, Bialik/Basel Sts., 84100 Beer Sheva, Israel;

Green Processes Center, Chemical Engineering Department, Sami Shamoon College of Engineering, Bialik/Basel Sts., 84100 Beer Sheva, Israel;

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正文语种 eng
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关键词
isoamyl acetate; lipase; triacetin; green solvent;

机译：乙酸异戊酯脂肪酶三醋精;绿色溶剂;

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1. Lipase Mediated Isoamyl Acetate Synthesis in Solvent-Free System Using Vinyl Acetate as Acyl Donor [J] . Kumari Annapurna, Mahapatra Paramita, Garlapati Vijay Kumar, Food Technology and Biotechnology . 2009,第1期

机译：脂肪酸介导的乙酸异戊酯的无溶剂体系合成
2. Lipase Mediated Isoamyl Acetate Synthesis in Solvent-Free System Using Vinyl Acetate as Acyl Donor [J] . Kumari Annapurna, Mahapatra Paramita, Garlapati Vijay Kumar, Food Technology and Biotechnology . 2009,第1期

机译：脂肪酸介导的乙酸异戊酯的无溶剂体系合成
3. Lipase Mediated Isoamyl Acetate Synthesis in Solvent-Free System Using Vinyl Acetate as Acyl Donor [J] . Kumari Annapurna, Mahapatra Paramita, Garlapati Vijay Kumar, Food Technology and Biotechnology . 2009,第1期

机译：脂肪酸介导的乙酸异戊酯的无溶剂体系合成
4. Deep Eutectic Solvents for Candida antarctica Lipase B-Catalyzed Reactions [C] . Johnathan T. Gorke, Friedrich Srienc, and Romas J. Kazlauskas Symposium on ionic liquid applications: pharmaceuticals, therapeutics, and biotechnology . 2010

机译：念珠菌抗野胶脂肪酶B催化反应的深层共晶溶剂
5. Enhancement of lipase catalyzed isoamyl acetate synthesis by high hydrostatic pressure and use of alternative solvents. [D] . Eisenmenger, Michael Joseph. 2009

机译：通过高静水压力和使用替代溶剂来增强脂肪酶催化的乙酸异戊酯的合成。
6. Analysis of the Conformational Stability and Activity of Candida antarctica Lipase B in Organic Solvents [O] . Cong Li, Tianwei Tan, Haiyang Zhang, 2010

机译：南极念珠菌脂肪酶B在有机溶剂中的构象稳定性和活性分析
7. Production of a biodiesel-like biofuel without glycerol generation, by using Novozym 435, an immobilized Candida antarctica lipase [O] . Luna Carlos, Verdugo-Escamilla Cristóbal, Sancho Enrique D., 2014

机译：使用固定化南极假丝酵母脂肪酶Novozym 435生产不含甘油的生物柴油样生物燃料

Glycerol triacetate as solvent and acyl donor in the production of isoamyl acetate with Candida antarctica lipase B

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