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Production of D-arabitol by a newly isolated Kodamaea ohmeri

机译：通过新分离的小木麻黄生产D-阿拉伯糖醇

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摘要

A new yeast, isolated from natural osmophilic sources, produces D-arabitol as the main metabolic product from glucose. According to 18S rRNA analysis, the NH-9 strain belongs to the genus Kodamaea. The optimal culture conditions for inducing production of D-arabitol were 37 ℃, neutral pH, 220 rpm shaking, and 5% inoculum. The yeast produced 81.2 ± 0.67 g L~(-1) D-arabitol from 200 g L~(-1) D-glucose in 72 h with a yield of 0.406 g g~(-1) glucose and volumetric productivity Q_p of 1.128 g L~(-1) h~(-1). Semi-continuous repeated-batch fermentation was performed in shaker-flasks to enhance the process of D-arabitol production by Kodamaea ohmeri NH-9 from D-glucose. Under repeated-batch culture conditions, the highest volumetric productivity was 1.380 g L~(-1) h~(-1).
机译：从天然渗透性来源中分离出来的一种新酵母从葡萄糖中产生D-阿拉伯糖醇作为主要代谢产物。根据18S rRNA分析，NH-9菌株属于Kodamaea属。诱导产生D-阿拉伯糖醇的最佳培养条件为37℃，中性pH，220rpm摇动和5％接种量。酵母在72小时内由200 g L〜（-1）D-葡萄糖产生了81.2±0.67 g L〜（-1）D-阿拉伯糖醇，葡萄糖产量为0.406 gg〜（-1），容积生产率Q_p为1.128 g L〜（-1）h〜（-1）。在摇瓶中进行半连续重复分批发酵，以增强欧姆木NH-9从D-葡萄糖生产D-阿拉伯糖的过程。在重复分批培养条件下，最高产量为1.380 g L〜（-1）h〜（-1）。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2010年第5期|P.565-571|共7页
  • 作者

    Hong-Yang Zhu; rnHong Xu; rnXiao-Yan Dai; Yang Zhang; rnHan-Jie Ying; rnPing-Kai Ouyang;

  • 作者单位

    State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing University of Technology, No. 5 Xinmofan Road, Gulou District, 210009 Nanjing, China;

    rnState Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing University of Technology, No. 5 Xinmofan Road, Gulou District, 210009 Nanjing, China;

    rnState Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing University of Technology, No. 5 Xinmofan Road, Gulou District, 210009 Nanjing, China;

    rnState Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing University of Technology, No. 5 Xinmofan Road, Gulou District, 210009 Nanjing, China;

    rnState Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Gulou District, 210009 Nanjing, China;

    rnState Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Gulou District, 210009 Nanjing, China;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    D-arabitol; kodamaea ohmeri; repeated-batch; osmophilic yeast;

    机译：D-阿拉伯糖醇;欧d重复批处理渗透酵母;

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