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Improvement of laccase production and its properties by low-energy ion implantation

机译：通过低能离子注入改善漆酶的生产及其性能

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摘要

Low-energy ion implantation was employed to breed laccase producing strain Paecilomyces sp. WSH-L07 and a mutant S152 that exhibited an activity of more than three times over the wild strain was obtained. The optimum substrate of both the wild and mutant laccases was 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate), and followed by guaiacol with optimal pH at 3.4 and 5.0, respectively, while the mutant laccase exhibited a broader active pH range. The mutant laccase had a higher optimal catalytic temperature (60-65 ℃) than the wild one (55 ℃), and the wild laccase deactivated rapidly when temperature increased above 55 ℃. Furthermore, the mutant laccase was more stable under neutral and alkaline conditions. A thermostability experiment revealed that the mutant laccase was superior to the wild laccase. Both laccases were stable in the presence of metal ions, mildly inhibited by SDS (0.5 mM), EDTA (1 mM) and 1,4-dithiothreitol (0.5 mM), and almost completely inhibited by 0.1 mM NaN_3.
机译：低能离子注入被用于繁殖产生漆酶的菌株Paecilomyces sp。获得了WSH-L07和突变体S152，其表现出超过野生菌株三倍以上的活性。野生漆酶和突变漆酶的最佳底物分别是2,2'-叠氮基双（3-乙基苯并噻唑啉-6-磺酸盐），其次是愈创木酚，其最佳pH分别为3.4和5.0，而突变漆酶则表现出更宽的范围。有效pH范围。突变漆酶的最佳催化温度（60-65℃）高于野生漆酶（55℃），当温度升高到55℃以上时，野生漆酶迅速失活。此外，突变漆酶在中性和碱性条件下更稳定。热稳定性实验表明，突变漆酶优于野生漆酶。两种漆酶在金属离子存在下均稳定，被SDS（0.5 mM），EDTA（1 mM）和1,4-二硫苏糖醇（0.5 mM）轻度抑制，而几乎完全被0.1 mM NaN_3抑制。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2010年第5期|P.639-646|共8页
  • 作者

    Zhiyu Liu; rnDongxu Zhang; rnZhaozhe Hua; Jianghua Li; rnGuocheng Du; rnJian Chen;

  • 作者单位

    Key Laboratory of Industrial Biotechnology, School of Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, 214122 Wuxi, Jiangsu, China;

    rnKey Laboratory of Industrial Biotechnology, School of Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, 214122 Wuxi, Jiangsu, China;

    rnKey Laboratory of Industrial Biotechnology, School of Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, 214122 Wuxi, Jiangsu, China;

    rnKey Laboratory of Industrial Biotechnology, School of Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, 214122 Wuxi, Jiangsu, China;

    rnKey Laboratory of Industrial Biotechnology, School of Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, 214122 Wuxi, Jiangsu, China;

    rnKey Laboratory of Industrial Biotechnology, School of Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, 214122 Wuxi, Jiangsu, China;

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  • 正文语种 eng
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  • 关键词

    laccase; paecilomyces sp.; ion implantation; yield; enzyme properties;

    机译：漆酶拟青霉离子注入让;酶性质;

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