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首页> 外文期刊>Bioprocess and Biosystems Engineering >Batch and fed-batch fermentation of Bacillus thuringiensis using starch industry waste water as fermentation substrate
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Batch and fed-batch fermentation of Bacillus thuringiensis using starch industry waste water as fermentation substrate

机译：以淀粉工业废水为发酵底物的苏云金芽孢杆菌分批补料发酵

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摘要

Bacillus thuringiensis var. kurstaki biopesticide was produced in batch and fed-batch fermentation modes using starch industry wastewater as sole substrate. Fed-batch fermentation with two intermittent feeds (at 10 and 20 h) during the fermentation of 72 h gave the maximum delta-endotoxin concentration (1,672.6 mg/L) and entomotoxicity (Tx) (18.5 × 10~6 SBU/mL) in fermented broth which were significantly higher than maximum delta-endotoxin concentration (511.0 mg/L) and Tx (15.8 × 10~6 SBU/mL) obtained in batch process. However, fed-batch fermentation with three intermittent feeds (at 10, 20 and 34 h) of the fermentation resulted in the formation of asporogenous variant (Spo-) from 36 h to the end of fermentation (72 h) which resulted in a significant decrease in spore and delta-endotoxin concentration and finally the Tx value. Tx of suspended pellets (27.4 × 10~6 SBU/mL) obtained in fed-batch fermentation with two feeds was the highest value as compared to other cases.
机译：苏云金芽孢杆菌变种以淀粉工业废水为唯一底物，分批和分批发酵两种方式生产了kurstaki生物农药。分批补料分批发酵在72小时的发酵过程中（分别在10和20小时）（在10和20小时）产生了最大的δ-内毒素浓度（1,672.6 mg / L）和肠毒素（Tx）（18.5×10〜6 SBU / mL）。发酵肉汤明显高于分批法获得的最大δ-内毒素浓度（511.0 mg / L）和Tx（15.8×10〜6 SBU / mL）。但是，分批补料发酵，在发酵的36小时到发酵结束（72 h）期间，采用三种间歇补料（分别在10、20和34 h）形成了孢子变体（Spo-）。降低孢子和δ-内毒素的浓度，最后降低Tx值。与其他情况相比，两次补料分批发酵获得的悬浮颗粒的Tx（27.4×10〜6 SBU / mL）最高。

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