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Chitooligosaccharides enzymatic production by Metarhizium anisopliae

机译：异僵菌酶法生产壳寡糖

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摘要

The products of chitosan hydrolysis are chitooligosaccharides and are used mainly for medical applications due to their specific biological activities. The objective of this study was to detect and identify the products of enzymatic hydrolysis of chitosan (dimers to hexamers) using a crude extract of chitosanolytic enzymes produced by the fungus Metarhizium anisopliae. These fungus was able to produce, during 48 h cultivation in a medium containing chitosan, chitooligosaccharides ranging from dimers, trimers, tetramers and pentamers at concentrations 0.2, 0.19, 0.06, 0.04 mg/mL, respectively, and the enzymatic activity was 2.5 U/L. Using the crude enzyme extract for chitosan hydrolysis, we detected the presence of dimers to hexamers at hydrolysis times of 10, 20, 30,40, 50 and 60 min of enzymatic reaction, but the yields were higher at 10 min (54%). The hexamers was obtained only with 30 min of reaction with concentration of 0.004 mg/mL.
机译：壳聚糖水解产物是壳寡糖，由于其特定的生物活性而主要用于医学应用。这项研究的目的是检测和鉴定壳聚糖分解酶产生的壳聚糖分解酶粗提物对壳聚糖的酶促水解产物（二聚体到六聚体）。在含有壳聚糖的培养基中培养48小时后，这些真菌能够分别生产浓度分别为0.2、0.19、0.06、0.04 mg / mL的二聚体，三聚体，四聚体和五聚体的壳寡糖，酶活性为2.5 U / L.使用粗酶提取物进行壳聚糖水解，我们在酶促反应的10、20、30、40、50和60分钟的水解时间检测到二聚体到六聚体的存在，但在10分钟时产率更高（54％）。仅在反应30分钟后即可获得六聚体，浓度为0.004 mg / mL。

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