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首页> 外文期刊>Bioprocess and Biosystems Engineering >Effect Of Culture Temperature On The Heterologous Expression Of Pleurotus Eryngii Versatile Peroxidase In Aspergillus Hosts
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Effect Of Culture Temperature On The Heterologous Expression Of Pleurotus Eryngii Versatile Peroxidase In Aspergillus Hosts

机译：培养温度对黑曲霉宿主中杏鲍菇侧耳多功能过氧化物酶异源表达的影响

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摘要

Production of recombinant versatile peroxidase in Aspergillus hosts was optimized through the modification of temperature during bioreactor cultivations. To further this purpose, the cDNA encoding a versatile peroxidase of Pleurotus eryngii was expressed under control of the alcohol dehydrogenase (alcA) promoter of Aspergillus nidulans. A dependence of recombinant peroxidase production on cultivation temperature was found. Lowering the culture temperature from 28 to 19 ℃ enhanced the level of active peroxidase 5.8-fold and reduced the effective proteolytic activity twofold. Thus, a maximum peroxidase activity of 466 U L~(-1) was reached. The same optimization scheme was applied to a recombinant Aspergillus niger that bore the alcohol dehydrogenase regulator (alcR), enabling transformation with the peroxidase cDNA under the same alcA promoter. However, with this strain, the peroxidase activity was not improved, while the effective proteolytic activity was increased between 3- and 11 -fold compared to that obtained with A. nidulans.
机译：通过在生物反应器培养过程中改变温度，优化了曲霉属宿主中重组通用过氧化物酶的生产。为了进一步实现该目的，在构巢曲霉的醇脱氢酶（alcA）启动子的控制下表达了编码杏鲍菇侧耳的通用过氧化物酶的cDNA。发现重组过氧化物酶生产对培养温度的依赖性。将培养温度从28℃降低到19℃，活性过氧化物酶水平提高了5.8倍，有效蛋白水解活性降低了两倍。因此，达到了466 U L〜（-1）的最大过氧化物酶活性。将相同的优化方案应用于带有酒精脱氢酶调节剂（alcR）的重组黑曲霉，从而能够在同一alcA启动子下用过氧化物酶cDNA进行转化。但是，与该菌株相比，过氧化物酶活性没有得到改善，而有效蛋白水解活性与构巢曲霉相比提高了3至11倍。

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