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首页> 外文期刊>Bioprocess and Biosystems Engineering >Effects of dissolved oxygen tension and agitation rate on the production of heat-shock protein glycoprotein 96 by MethA tumor cell suspension culture in stirred-tank bioreactors
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Effects of dissolved oxygen tension and agitation rate on the production of heat-shock protein glycoprotein 96 by MethA tumor cell suspension culture in stirred-tank bioreactors

机译：搅拌罐生物反应器中MethA肿瘤细胞悬浮培养的溶解氧张力和搅拌速率对热休克蛋白糖蛋白96产生的影响

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摘要

Heat-shock protein glycoprotein (gp96) serves as a natural adjuvant for chaperoning antigenic peptide into the immune surveillance pathway. In our laboratory, MethA tumor cell suspension culture process has been recently developed for gp96 production in spinner flask. In this work, effects of dissolved oxygen tension (DOT) and agitation rate on this process were studied in stirred-tank bioreactor. The optimal conditions for gp96 production were different with those for MethA tumor cell growth. MethA tumor cell growth pattern was not much changed by various levels of DOT and agitation rate, while gp96 biosynthesis was more sensitive to DOT and agitation rate. Compared with 50% of DOT, the production and specilie-productivity of gp96 was increased by 27 and 66% at 10% of DOT, respectively. Compared with the agitation rate of 100 rpm, the production and volumetric productivity of gp96 was increased by 48 and 144% at the agitation rate of 200 rpm, respectively. Low DOT (i.e., 10% of air saturation) and high agitation rate (i.e., 200 rpm) were identified to be favorable for gp96 biosynthesis. The results of thisrnwork might be useful to scale-up the bioprocess into the pilot scale.
机译：热休克蛋白糖蛋白（gp96）作为天然佐剂，可将抗原性肽带入免疫监视途径。在我们的实验室中，最近已经开发出MethA肿瘤细胞悬浮培养方法，用于在旋转瓶中生产gp96。在这项工作中，在搅拌釜式生物反应器中研究了溶解氧张力（DOT）和搅拌速率对该过程的影响。 gp96产生的最佳条件与MethA肿瘤细胞生长的最佳条件不同。 MethA肿瘤细胞的生长方式在不同水平的DOT和搅拌速率下变化不大，而gp96的生物合成对DOT和搅拌速率更敏感。与DOT的50％相比，在10％的DOT下，gp96的产量和专一性分别提高了27％和66％。与100 rpm的搅拌速度相比，gp96的产量和体积生产率在200 rpm的搅拌速度下分别增加了48％和144％。较低的DOT（即空气饱和度的10％）和较高的搅拌速度（即200 rpm）被认为对gp96生物合成有利。这项工作的结果可能有助于将生物过程扩大到试验规模。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2009年第4期|475-484|共10页
  • 作者

    Ya-Jie Tang; Hong-Mei Li; Jean-Francois P. Hamel;

  • 作者单位

    Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA Hubei Provincial Key Laboratory of Industrial Microbiology. College of Bioengineering, Hubei University of Technology, 430068 Wuhan. China National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100080 Beijing, China;

    Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA Hubei Provincial Key Laboratory of Industrial Microbiology. College of Bioengineering, Hubei University of Technology, 430068 Wuhan. China;

    Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA;

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  • 正文语种 eng
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  • 关键词

    heat-shock protein glycoprotein 96; MethA tumor cell suspension culture; stirred-tank bioreactor; process development; dissolved oxygen tension; agitation rate;

    机译：热激蛋白糖蛋白96;MethA肿瘤细胞悬浮培养;搅拌釜式生物反应器工艺开发;溶解氧张力搅拌速度;

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