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首页> 外文期刊>Bioprocess and Biosystems Engineering >Electrochemical immune-biosensor for immunoglobulin G based bioelectrocatalytic reaction on micro-comb electrodes
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Electrochemical immune-biosensor for immunoglobulin G based bioelectrocatalytic reaction on micro-comb electrodes

机译：电化学免疫生物传感器，用于微梳状电极上基于免疫球蛋白G的生物电催化反应

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摘要

A new amplification strategy of electrochemical signaling from antigen-antibody interactions was proposed via back-filling immobilization of horseradish peroxidase (HRP), immunoglobulin G antibodies (anti-IgG) and gold nanoparticles onto a three-dimensional sol-gel (3DSG)-functionalized biorecognition interface. The 3DSG sol-gel network was employed not only as a building block for the surface modification but also as a matrix for ligand functionalization. The signal-amplification was based on the bioelectrocatalytic reaction of the back-filling immobilization of HRP to H_2O_2. With the non-competitive format, the formation of the antigen-antibody complex by a simple one-step immunoreaction between the immobilized anti-IgG and IgG in sample solution inhibited partly the active center of HRP, and decreased the immobilized HRP towards H_2O_2 reduction. Under optimal conditions, the proposed immunosensor exhibited a good electrochemical behavior to IgG in a dynamic range of 1.12-162 ng/mL with a detection limit of 0.56 ng/mL (at 3(5). Moreover, the precision, reproducibility and stability of the as-prepared immunosensor were acceptable. Importantly, the proposed methodology would be valuable for diagnosis and monitoring of biomarkers and its metastasis.
机译：通过将辣根过氧化物酶（HRP），免疫球蛋白G抗体（抗IgG）和金纳米颗粒回填固定化到功能化的三维溶胶-凝胶（3DSG）上，提出了一种新的从抗原-抗体相互作用中电化学信号传递的扩增策略。生物识别界面。 3DSG溶胶-凝胶网络不仅用作表面改性的基础，而且还用作配体功能化的基质。信号放大基于HRP回填固定到H_2O_2的生物电催化反应。在非竞争形式下，样品溶液中固定的抗IgG和IgG之间通过简单的一步免疫反应形成抗原-抗体复合物，部分抑制了HRP的活性中心，并使固定的HRP降低了H_2O_2。在最佳条件下，提出的免疫传感器在1.12-162 ng / mL的动态范围内对IgG表现出良好的电化学行为，检出限为0.56 ng / mL（在3（5）时）。重要的是，所提出的方法对于生物标志物的诊断和监测及其转移将是有价值的。

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