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首页> 外文期刊>Bioprocess and Biosystems Engineering >Comparative profiles of α-amylase production in conventional tray reactor and GROWTEK bioreactor
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Comparative profiles of α-amylase production in conventional tray reactor and GROWTEK bioreactor

机译：常规塔板反应器和GROWTEK生物反应器中α-淀粉酶生产的比较曲线

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摘要

GROWTEK bioreactor was used as modified solid-state fermentor to circumvent many of the problems associated with the conventional tray reactors for solid-state fermentation (SSF). Aspergillus oryzae IFO-30103 produced very high levels of α-amylase by modified solid-state fermentation (mSSF) compared to SSF carried out in enamel coated metallic trays utilizing wheat bran as substrate. High α-amylase yield of 15,833 U g~(-1) dry solid in mSSF were obtained when the fungus were cultivated at an initial pH of 6.0 at 32℃ for 54 h whereas α-amylase production in SSF reached its maxima (12,899 U g~(-1) dry solid ) at 30℃ after 66 h of incubation. With the supplementation of 1% NaNO_3, the maximum activity obtained was 19,665 U g~(-1) dry solid (24% higher than control) in mSSF, whereas, in SSF maximum activity was 15,480 U g~(-1) dry solid in presence of 0.1% Triton X-100 (20% higher than the control).
机译：GROWTEK生物反应器被用作改良的固态发酵罐，以规避与固态发酵常规托盘反应器（SSF）相关的许多问题。与在以麦麸为底物的搪瓷涂层金属托盘中进行的SSF相比，米曲霉IFO-30103通过改良的固态发酵（mSSF）产生了非常高水平的α-淀粉酶。当真菌在32℃初始pH 6.0下培养54小时时，在mSSF中获得了15,833 U g〜（-1）干燥固体的高α-淀粉酶产量，而在SSF中α-淀粉酶的产量达到最大值（12,899 U孵育66小时后，在30℃下干燥至g〜（-1）干燥固体。补充1％NaNO_3，在mSSF中获得的最大活性为19,665 U g〜（-1）干燥固体（比对照高24％），而在SSF中最大活性为15,480 U g〜（-1）干燥固体。在存在0.1％Triton X-100的情况下（比对照高20％）。

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