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首页> 外文期刊>Bioprocess and Biosystems Engineering >Immobilization of urease by using chitosan-alginate and poly(acrylamide-co-acrylic acid)/κ-carrageenan supports
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Immobilization of urease by using chitosan-alginate and poly(acrylamide-co-acrylic acid)/κ-carrageenan supports

机译：通过使用壳聚糖-海藻酸酯和聚（丙烯酰胺-共丙烯酸）/κ-角叉菜胶载体固定尿素酶

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摘要

Jack bean urease (urea aminohydrolase, E.C. 3.5.1.5) was entrapped into chitosan-alginate polyelectrolyte complexes (C-A PEC) and poly(acryl-amide-co-acrylic acid)/κ-carrageenan (P(AAm-co-AA)/carrageenan) hydrogels for the potential use in immobilization of urease, not previously reported. The effects of pH, temperature, storage stability, reuse number, and thermal stability on the free and immobilized urease were examined. For the free and immobilized urease into C-A PEC and P(AAm-co-AA)/carrageenan, the optimum pH was found to be 7.5 and 8, respectively. The optimum temperature of the free and immobilized enzymes was also observed to be 55 and 60℃, respectively. Michaelis-Menten constant (K_m) values for both immobilized urease were also observed smaller than free enzyme. The storage stability values of immobilized enzyme systems were observed as 48 and 70%, respectively, after 70 days. In addition to this, it was observed that, after 20th use in 5 days, the retained activities for immobilized enzyme into C-A PEC and P(AAm-co-AA)/carrageenan matrixes were found as 55 and 89%, respectively. Thermal stability of the free urease was also increased by a result of immobilization.
机译：将杰克豆脲酶（尿素氨基水解酶，EC 3.5.1.5）截留在壳聚糖-海藻酸酯聚电解质复合物（CA PEC）和聚（丙烯酰胺-共丙烯酸）/κ-角叉菜胶（P（AAm-co-AA）/角叉菜胶水凝胶可用于固定尿素酶，尚未见报道。研究了pH，温度，储存稳定性，再利用次数和热稳定性对游离和固定化脲酶的影响。对于游离和固定化的脲酶到C-A PEC和P（AAm-co-AA）/角叉菜胶中，最佳pH分别为7.5和8。游离和固定化酶的最适温度分别为55和60℃。还观察到两种固定的脲酶的Michaelis-Menten常数（K_m）值均小于游离酶。 70天后，固定化酶体系的储存稳定性值分别为48％和70％。除此之外，还发现在5天的20次使用后，固定化酶在C-A PEC和P（AAm-co-AA）/角叉菜胶基质中的固定保留活性分别为55％和89％。固定的结果也增加了游离脲酶的热稳定性。

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