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首页> 外文期刊>Bioprocess and Biosystems Engineering >Fully integrated L-phenylalanine separation and concentration using reactive-extraction with liquid-liquid centrifuges in a fed-batch process with E. coli
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Fully integrated L-phenylalanine separation and concentration using reactive-extraction with liquid-liquid centrifuges in a fed-batch process with E. coli

机译：在大肠杆菌的分批补料过程中，使用液-液离心机通过反应萃取进行L-苯丙氨酸的完全分离和浓缩

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摘要

A novel in situ product recovery (ISPR) approach for the (fully) integrated separation of L-phenylalanine (L-phe) from a 20 1 fed-batch process with the recombinant L-tyrosine auxotrophic strain E. coli F-4/pF81 is presented. The strain was rationally constructed for the production of the aromatic amino acid. Glucose and tyrosine control is used. A reactive extraction system consisting of kerosene, the cation-selective carrier D_2EHPA and sulphuric acid, all circulating in liquid-liquid centrifuges, is applied for the on-line L-phe separation from cell- and protein-free permeate. Permeate is drained off from the bioreactor bypass. Using the novel ISPR approach, a significantly extended product formation period at 0.25 mmol/(g~*h) together with a reduced by-product formation and a 28% relative glucose/L-phe yield increase is observed. Thus, the ISPR approach is superior to the reference non-ISPR process and even offers extraction rates approximately three times higher than the published membrane-based process.
机译：一种新颖的原位产物回收（ISPR）方法，用于从20 1分批补料工艺中用重组L-酪氨酸营养缺陷型菌株E. coli F-4 / pF81完全分离L-苯丙氨酸（L-phe）。被表达。为产生芳香族氨基酸合理地构建了菌株。使用葡萄糖和酪氨酸控制。一种由煤油，阳离子选择性载体D_2EHPA和硫酸组成的反应萃取系统，均在液-液离心机中循环，用于从无细胞和蛋白质的渗透物中在线分离L-phe。渗透物从生物反应器旁路中排出。使用新颖的ISPR方法，观察到在0.25mmol /（g·h）下产物形成时间显着延长，同时副产物形成减少，相对葡萄糖/ L-phe产量增加28％。因此，ISPR方法优于参考非ISPR方法，甚至可以提供比已发表的基于膜的方法高大约三倍的提取率。

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