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首页> 外文期刊>Bioprocess and Biosystems Engineering >Purification of lysozyme by multistage affinity filtration
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Purification of lysozyme by multistage affinity filtration

机译：通过多阶段亲和过滤纯化溶菌酶

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摘要

A multistage affinity filtration process was developed for the purification of proteins. An affinity adsorbent was prepared by immobilizing Cibacron Blue 3GA to TSK gel HW-65F. Adsorption equilibrium experiments showed that the blue TSK gel had a high affinity for lysozyme, while its binding to bovine serum albumin (BSA) was weaker. Using a three-stage affinity filtration system, lysozyme was purified from a model system (a mixture of lysozyme and BSA) and a natural source (chicken egg white). From the chicken egg white, the three-stage affinity filtration increased the recovery yield of lysozyme from 61 to 96%, compared with the one-stage process. A mathematical model taking into account the film and interior diffusions of protein and eluant was developed for the modeling and analysis of the experimental data. Both the experimental and modeling results indicate that the multistage affinity filtration technique can be employed for the selective recovery of proteins.
机译：开发了用于蛋白质纯化的多级亲和过滤方法。通过将Cibacron Blue 3GA固定在TSK凝胶HW-65F上制备亲和吸附剂。吸附平衡实验表明，蓝色TSK凝胶对溶菌酶具有很高的亲和力，而与牛血清白蛋白（BSA）的结合却较弱。使用三阶段亲和过滤系统，从模型系统（溶菌酶和BSA的混合物）和天然来源（鸡蛋白）中纯化溶菌酶。与一阶段过程相比，三阶段亲和过滤从鸡蛋白中将溶菌酶的回收率从61％提高到96％。考虑到蛋白质和洗脱液的膜和内部扩散，建立了一个数学模型，用于对实验数据进行建模和分析。实验和建模结果均表明，多级亲和力过滤技术可用于蛋白质的选择性回收。

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